FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001386322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001386322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9304474
Sequences flagged as poor quality0
Sequence length126
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTAGAGTAGTTCATGCTTCTGACAAAACCGTGGAAGTAAAACCAGGCA136830.1470582861535214No Hit
GATGTGGGGAGTTCCTTCCTTTCTGAGCCTCCATCTCCCTTGGCTTTTGG126780.1362570307574614No Hit
CCCTTACATCTGTGCTTTCATTGCTGGTGTTTTCCTCTCGTTTTCTCTCT125230.13459116549737257No Hit
GATGTTGACTGAGAGGCTGTGCCGTTACTGCTACTCTCTTCTTCCTCTTT115550.12418756826017247No Hit
AGGAGGCACATGTTTAACTCAGGGTATCCCTCAAATCTGAAATCAAACAC104330.11212885328069055No Hit
GCCCCACTCTACGTCAGCAATTCCCTCAAGTTTTCTATTCCCACCCTCAC101950.10957094404261865No Hit
ATGAATTCATCCCTGTAAATGTACCCACAACGGTTCATTAGGAAAAGCAC96400.10360607165971983No Hit
TATCCTTCTTGTCCCCAGTGTCCAGCTCAGGATCAGTATATGCCAGCAGG95820.10298271562691239No Hit
AGGGGAGACTCCTCAGGTGGAGGGGACAGAGGAGACTCTTCAAATGGTGG95440.10257430995024544No Hit
TTAGTAGGTTTAAAAATGTCTTCTGGAGTTCTCTCCCGTATCTTCACAGC93180.1001453709258578No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)