FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001386443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001386443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6526374
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAATAAGAGCAGCCTGAGTAGGGGTAACAAGAAGAAACCCGGGATGCC112260.17200975610653021No Hit
GGCGAGAATAAGGGTCAGGGGACTGGAAGCGAGGGGTAACGGGTGATGGG107370.1645170810008743No Hit
AGCTTGAGAAACACTGAAGGGGTACCATTTACCACCACATCCTACTGGAT103250.15820423408158957No Hit
ATCCTACTGGATTACAAACGCTAGATCTTTGGATCTCCACGACTAGCAAG102490.15703972833919722No Hit
AAACGTTTTGAATCACATGACGCATTGTCAGGCTGGGAAAGCCTGCCAAG97370.14919463702202784No Hit
TGATGCCCTTGGTCTTCTGTGGAGTCTATTCTTCTAATCGGGTTGTTCTC97090.14876560859062016No Hit
CCCACGTCCCCACCACCTGAGGCATCACGCCTGTCACCACCACCTGAGGA92840.1422535698996104No Hit
CTGCCCACTGGCCCTGACTACTATTCCCAGCTGCTTACCAAGGTCAGAAA86470.1324931730850852No Hit
TTTTTTTAAAATGAGAGATGAAAGAAATAAAGCATAACCCAAATTCAAAG83710.1282641785469236No Hit
TTTATTTAGCAAGTTGTAATTCTGTTTTCCTGTGGGTGCTTTTCCTAATG83490.127927084779389No Hit
CAAGGTGTGGACGCACACACAGACTTCTACAAGTGACTCTGGTCTTGTGG81830.12538355907890048No Hit
TGACAAAAGCCACCACCTTCCTTCAGCGCCGGGTACCTTGTAGTCATGGA80850.12388195956897351No Hit
GAGCTTTAGCACCCAACCTACCCGAAGTACCCAGAAGTCCCCTCACCGGA79770.1222271356192581No Hit
AGGGAAGGGAGTGGGCAAAACAGGCATTGGTCAGACAGCAAAGACTAAAC78990.12103198498890809No Hit
AGTGCAAAGGAATTGCCTTATTTCGAGGGTGATTTCTGGCCCAATGTTCT78560.12037311989781768No Hit
CAATGTTCTGGAAGAAAGCATTAAGGAACTGGAACAGGAGGAAGAAGAGA78150.11974489969468498No Hit
GGCCAGAGAAGATATGATCTGAGGTGCCCAGCCTAGGAATCCACATTTAG77940.11942312837112921No Hit
TGGAAAGAGGAGCTTTGGAGATTCTGAATTGATCTTAGGATGGAAAAATA77220.11831991240465227No Hit
TGGAACAGCCACTCTTCATACTGCCGCTGGTCAGCTTCATCTGGGAAAAG75190.11520945627694643No Hit
CTGGACGTTCCCCAAGGACATGGGGTTAAATGAATTCATCCCTGTAAATG73680.11289576723614063No Hit
GTGAGTATGGGGCGTGGGAGGAAGGTGCAGGGGAGTGGTTTGGGTCATAT73630.1128191550162464No Hit
AGTCTCTGCCAGCTTTCAAGACATTTTAAGCTTTCATGTTTCTTGTCAGC72640.11130223306234058No Hit
TCTCGTTTTCTCTCTTCTTCCTCCTGTTCCAGTTCCTTAATGCTTTCTTC72500.11108771884663675No Hit
GTACCATTTACCACCACATCCTACTGGATTACAAACGCTAGATCTTTGGA72430.11098046173878481No Hit
CCTAGGAATCCACATTTAGGGAATGGCAGAGAAGTGGTGAAAGGAGGAGG71800.1100151477681175No Hit
GCTGCCTGCAGGAAACCCCAGTGTTTGATCTTGTCATTACCCTGATCTGT71790.10999982532413864No Hit
AGCATTAAGGAACTGGAACAGGAGGAAGAAGAGAGAAAACGAGAGGAAAA71750.10993853554822326No Hit
CAGTCTGCATCCCAAACAAGCTCCCCTGAAACAAGCCCGTTGTACACTAA71660.10980063355241364No Hit
CTTTAAAAGAACATAGAAATTCCTATATGTACATGCATGTTTTCACAGGA71640.10976998866445593No Hit
ATAAAACATGTTTTCTTATTTTAAAACAAGGACAATTTCAAAGGATCAGT71420.10943289489692132No Hit
AGAACCCTTTATTTTTTACTGTATGTTTATTCCGTGAAATAATGGGTATT70840.10854419314614823No Hit
TTGAGTATGTGACAGAGGCTGGGTTTAGGAGGCCAGGCTGGTGGCTTGGG70300.10771678117129052No Hit
CCTGGATGTTTCTGTCGATGAGGGCTTTGTGGATGATCTCCCGAAACTGA69890.10708856096815782No Hit
TGATAGGAGCAGGAAAACAGAGCTTTAGCACCCAACCTACCCGAAGTACC69340.10624582654932126No Hit
GTGTTCGTGCTGAATGCCCCATTAGGTTAAGATCATAGAAACGTGGCAGT68500.10495874125509817No Hit
GGGCTTTGCTATGATACTCGGGTAGGTTTTTCCCCCTAATTATCTACTAA67930.10408536194830391No Hit
AAAAAGAAATAGCTATATACTTACGGTTCGGGGTCTAAGGTATCATTTTT67910.10405471706034623No Hit
ACAGATCCTGAAAATGAGATGAAGGAGAAGCTCTTAAAAGAGTACTTAAT67820.1039168150645366No Hit
ATCCAAACTGCTATGCAAAAGGTAGGTACCTTTGACGTGAGAATTGGAAC67610.10359504374098083No Hit
CAGAGCTTTAGCACCCAACCTACCCGAAGTACCCAGAAGTCCCCTCACCG67500.1034264968572135No Hit
TGCTTCTCCATGGTGGCATATAGTTTCTGTGAGAGGTCGTTAGATACATT67450.10334988463731928No Hit
GGACACGTGGGCAATGGAGTTCAGAGAAGGGTCTGTACCTCAGTGGTTTC67270.10307408064570003No Hit
AACCTTACTAGACAAAGTGTGTTTGAGTCACTTCAGTAGATGGATGAAGT67230.10301279086978465No Hit
GTGAGGGGACTTCTGGGTACTTCGGGTAGGTTGGGTGCTAAAGCTCTGTT66830.1023998931106308No Hit
CCCCTGAGCATTGTCAGTGTTGGAGGCTGTGACCCGTACCTCTTTGTGTA66390.10172570557556156No Hit
AGGATGTTGCAAAGGACAAATATGTGTATTTCTTCCAGTGAGTTGTACTG66210.10144990158394232No Hit
AACCCTTTATTTTTTACTGTATGTTTATTCCGTGAAATAATGGGTATTTT66190.10141925669598464No Hit
GGGTGTTGTGTGCAAGATGGCATAGGGAGACTGATATAATCTCCTGGCCC66070.10123538736823848No Hit
CTTAAAAAATACAAGTAAGTTCTCTGCACAGGAAATTGGTTTAATGTAAT66070.10123538736823848No Hit
AAGAAGGGCTCTTAGATTAGATGTGCCATGAAGAGTTACAGCTGTTCCAG65770.10077571404887309No Hit
CAGCCTGCCACGTCAGATGGTGCCAGCAATAGATGCTAGAGAATAAAACA65440.10027007339757114No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)