FastQCFastQC Report
Fri 30 Dec 2022
EGAF00001407718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001407718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335379486
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3652474710.890572776416027TruSeq Adapter, Index 1 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC56428211.6825182324955916TruSeq Adapter, Index 1 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA34354541.0243482810991011TruSeq Adapter, Index 1 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAG29486880.8792094099637329TruSeq Adapter, Index 1 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAT20709650.6174990082726766TruSeq Adapter, Index 1 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15906110.47427200123981345TruSeq Adapter, Index 1 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGA15840930.47232853114933815TruSeq Adapter, Index 1 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA10257180.3058380261218481TruSeq Adapter, Index 1 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATA9803450.2923091724220723TruSeq Adapter, Index 1 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA9500980.28329043357171824TruSeq Adapter, Index 1 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACC7864990.23451016917594059TruSeq Adapter, Index 1 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7673180.22879097620180622TruSeq Adapter, Index 1 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAA6224170.18558588881610966TruSeq Adapter, Index 1 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGC3798630.11326363592792912TruSeq Adapter, Index 1 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACT3494790.10420404782897187TruSeq Adapter, Index 1 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACG3372910.10056995555178351TruSeq Adapter, Index 1 (100% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)