FastQCFastQC Report
Wed 6 Sep 2023
EGAF00001411784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001411784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74870044
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTC12266391.6383575252072777TruSeq Adapter, Index 8 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC10869121.451731482887869TruSeq Adapter, Index 8 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC4749110.6343137717402704TruSeq Adapter, Index 8 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCG4083080.5453556298163789TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)