FastQCFastQC Report
Sat 1 Apr 2023
EGAF00001416156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001416156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28058745
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC677120.2413222686902069No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT638780.22765807950426864No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA539150.19215043295771067No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT448840.15996438899886647TruSeq Adapter, Index 13 (97% over 40bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT412940.14716980392387471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)