FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001416161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001416161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21072941
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC421440.19999106911560183No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT346700.16452378431657924No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA267110.12675496979752374No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC242650.11514766733319284TruSeq Adapter, Index 3 (100% over 50bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT224510.10653947163805945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)