FastQCFastQC Report
Wed 14 Sep 2022
EGAF00001416171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001416171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24888773
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC651830.2618972016017021TruSeq Adapter, Index 6 (100% over 50bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC516310.20744694806770908No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT478850.19239598512952005No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA396650.15936904563354728No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT280380.11265320311290557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)