FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001416204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001416204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16969497
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT446600.26317810127194696TruSeq Adapter, Index 18 (97% over 40bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC381850.2250214016361239No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT248840.1466395851332541No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA205440.12106428375572946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)