FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001416207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001416207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19955625
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC555740.2784878950170691TruSeq Adapter, Index 4 (100% over 50bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC444670.22282940273732343No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT351930.17635629051959034No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA298980.14982241849102695No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT225060.11278023113783708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)