FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001416217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001416217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15397032
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC359460.2334605786361943No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT273240.1774627733448888No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT224360.1457163952117525TruSeq Adapter, Index 14 (97% over 44bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA221800.1440537371098534No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT160850.10446818581659113No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)