FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15770
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT480430.462904248573242No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT169010.716550412175016RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT12127.685478757133798RNA PCR Primer, Index 27 (96% over 25bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG11267.140139505389981RNA PCR Primer, Index 27 (96% over 30bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT4753.0120481927710845RNA PCR Primer, Index 27 (96% over 28bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA3892.466708941027267RNA PCR Primer, Index 27 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGT3362.1306277742549145No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG2881.8262523779327837RNA PCR Primer, Index 27 (100% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC1691.0716550412175017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.9511731135066582No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGC1460.9258084971464806TruSeq Adapter, Index 27 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC1440.9131261889663919No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGT1350.8560558021559923No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT1330.8433734939759037RNA PCR Primer, Index 27 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.7545973367152822No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTT1070.6785034876347495No Hit
CTTTACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC740.46924540266328474RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTACCTCGTATGCCGT690.4375396322130628No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGGACTCC660.4185161699429296No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTTCT640.40583386176284086RNA PCR Primer, Index 38 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.3297400126823082No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGTCTT500.31705770450221943No Hit
CTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC470.29803424223208624No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATATCGTATGCCGT390.24730500951173112No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGGACTC380.24096385542168677No Hit
ATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTT370.23462270133164237RNA PCR Primer, Index 27 (100% over 27bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACGGACTCC320.20291693088142043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC320.20291693088142043No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGTCTTCT290.18389346861128728RNA PCR Primer, Index 38 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.17755231452124287Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC280.17755231452124287No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTACCTCGTATGCCGTCTT270.1712111604311985No Hit
GTACATGGGTGGGAGCTGCAGACTGGAGCTGTTCCTATTCAGCCATCTTG200.12682308180088775No Hit
TCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGTCTTCTGCTTG190.12048192771084339RNA PCR Primer, Index 38 (96% over 26bp)
CTTATAACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG180.11414077362079898No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCCT180.11414077362079898RNA PCR Primer, Index 27 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC180.11414077362079898No Hit
TCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTTCTGCTTG170.1077996195307546RNA PCR Primer, Index 38 (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC170.1077996195307546No Hit
CTTTTATTCCATGGAAATACAGTAAGATTAAGAAATGCCATTTATAGGAG160.10145846544071022No Hit
TCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAA160.10145846544071022RNA PCR Primer, Index 27 (100% over 28bp)
CATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTTCTGCT160.10145846544071022RNA PCR Primer, Index 38 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGTCTTCTGCT160.10145846544071022RNA PCR Primer, Index 38 (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)