FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63388
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT40936.457058118255821No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT13712.1628699438379506TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT10131.598094276519215RNA PCR Primer, Index 1 (95% over 23bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG8011.263646115984098TruSeq Adapter, Index 1 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7061.1137754780084559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5500.8676721145958225No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGAACTCGTATGCCGT4010.6326118508234997No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT3380.5332239540607054TruSeq Adapter, Index 1 (96% over 26bp)
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA2970.4685429418817442RNA PCR Primer, Index 1 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2950.4653877705559412No Hit
GATATAGCAGGCTTAATGGTTCTATATTTTCAAAAGTTTTTAAGAATGGT2430.3833533160850634No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG2380.37546538777055594TruSeq Adapter, Index 1 (95% over 24bp)
GTACATGGGCAGGACCCCAGAACTGTGGAGTCAGTGGCAGCATGCAGCGC2090.32971540354641254No Hit
GTGTTAGACTTGCTGAGTCCTAGCTGTGTTTAGAACATCTCTATTCTACA1960.30920678992869316No Hit
GTTCTATATTTTCAAAAGTTTTTAAGAATGGTTTCTAACGTAGGAGAGGG1690.2666119770303527No Hit
ACATTTACCTTGGTCAAATTTGAACTGCTGCCATAGGTTTTGGGTGTAAA1630.25714646305294375No Hit
GTACATGGGGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA1600.25241370606423924No Hit
GTTTAGAACATCTCTATTCTACATTTACCTTGGTCAAATTTGAACTGCTG1580.2492585347384363No Hit
GTAAAAAAGGTTATGTTTTATTAATTGCTGGACAACCGTGGGAAAACAAA1490.2350602637723228No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC1480.23348267810942136No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGAACTCGTATGCCGTCTT1440.22717233545781534No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGAGCTCGTATGCCGT1400.22086199280620938No Hit
ATTTTAGTGTGTTAGACTTGCTGAGTCCTAGCTGTGTTTAGAACATCTCT1370.2161292358175049No Hit
CCCCAGAACTGTGGAGTCAGTGGCAGCATGCAGCGCCCCCTTGGGAAAGC1360.2145516501546034No Hit
GCATTAAGATTACCTTCAAATGAAAAAAAAAATCTCAAAATTTCTATTTG1340.21139647882880042No Hit
TCTCTATTCTACATTTACCTTGGTCAAATTTGAACTGCTGCCATAGGTTT1340.21139647882880042No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT1320.2082413075029974No Hit
GGCTTAATGGTTCTATATTTTCAAAAGTTTTTAAGAATGGTTTCTAACGT1310.20666372184009593No Hit
GTCCTAGCTGTGTTTAGAACATCTCTATTCTACATTTACCTTGGTCAAAT1300.20508613617719443No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC1300.20508613617719443No Hit
GTTAAAGGGTCATGGGAGAGAAAAGGTATCTGTGGGAAGAGAATAATCTC1280.20193096485139145No Hit
GCTTTTATTTTACTTCAAAGTTTACCTCAGATCAGCCTGGGAAGGTGAGG1280.20193096485139145No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT1260.19877579352558847No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT1240.19562062219978543No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1160.18299993689657348No Hit
ATTCTACATTTACCTTGGTCAAATTTGAACTGCTGCCATAGGTTTTGGGT1150.18142235123367198No Hit
TTTTTATTCTCTTGCTCTGAAAACTTGTATCAAAAAAAAAGAATTTAAAG1130.178267179907869No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1130.178267179907869No Hit
GTACATGGGATCTCTTCGAAAGGCTCACTCTCAAGCACGACTAAGAGCCT1120.1766895942449675No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA1110.17511200858206602No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC1060.16722408026755853Illumina PCR Primer Index 5 (95% over 24bp)
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT1060.16722408026755853No Hit
GTACATGGGGGGTATTTAAATTAGCATTTGTGAATTTAATAGCATTAAGA1050.16564649460465702No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC1010.15933615195305106No Hit
GTACATGGGACTCTAAAGTGATACTCAGAACAAAAGAAAGTCATAAAACT980.15460339496434658No Hit
GAGTACATGGGGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCC980.15460339496434658No Hit
CTCAAACACAGCTTTTATTTTACTTCAAAGTTTACCTCAGATCAGCCTGG970.15302580930144505No Hit
GCCATAGGTTTTGGGTGTAAAGAATGTTTACTGCCCTCCATTTAAATTCT960.15144822363854357No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.14987063797564207No Hit
ACGTAGGAGAGGGAAAACATCCACCATCCCTTTTCAGAATTTAAATGGAG900.14198270966113458No Hit
GCTATATCTGAGCAAATTAGTGGGTACCTTTTTTTTCTTATTTAAAGCAC870.13724995267243012No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT860.13567236700952862No Hit
GGCTTATGCAGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC860.13567236700952862No Hit
ATATTAGTGTCTTCTCTTCTGAAGATGTGATTCTTTTAATGCCATGTTCT830.13093961002082413No Hit
GTACATGGGGCATGGAGACTTGAGGAGGGAGGGCTTGAGGTTGGTGAGGT830.13093961002082413No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCAGAGGAG820.12936202435792263No Hit
GTATCAACGCAGAGTACATGGGCAGGACCCCAGAACTGTGGAGTCAGTGG820.12936202435792263No Hit
GTATTATCAAAACACATGTGCACACAGATTATTTTTTGGCTCCAAAACTG820.12936202435792263No Hit
GAATGTTTACTGCCCTCCATTTAAATTCTGAAAAGGGATGGTGGATGTTT800.12620685303211962No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGAACTCGTATGCCGTCTTCT790.12462926736921814RNA PCR Primer, Index 5 (95% over 21bp)
ATATAGAACCATTAAGCCTGCTATATCTGAGCAAATTAGTGGGTACCTTT790.12462926736921814No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.12462926736921814Clontech SMART CDS Primer II A (100% over 21bp)
TGCCTGTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTG780.12305168170631665No Hit
CTATTCTACATTTACCTTGGTCAAATTTGAACTGCTGCCATAGGTTTTGG780.12305168170631665No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA770.12147409604341516No Hit
CTCCTACGTTAGAAACCATTCTTAAAAACTTTTGAAAATATAGAACCATT770.12147409604341516No Hit
GTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCCTGGTGATA770.12147409604341516No Hit
TGTGCACACAGATTATTTTTTGGCTCCAAAACTGGATTGCAAAAGAAAGA760.11989651038051366No Hit
CACTGGAGCAGGGATTGGGCCCCCAAGGCCTCAGGTAGCCCCGTGCCTGT740.11674133905471068No Hit
GTACATATCATGTCAGTTTAGTAATCAATTATGTTGATATTGTTAAACTG740.11674133905471068No Hit
GTTTAATAACACATGATTTTTATTCTCTTGCTCTGAAAACTTGTATCAAA720.11358616772890767No Hit
GTACATGGGAGGCTCGGATGAGGTGCAGGTTGGACAGCAGCGCACGTTCA710.11200858206600618No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA710.11200858206600618No Hit
GTGTTGCTGAGTGACACTCAAGTAACTGGTGGTTTTTCCTGTGCAGCTGG710.11200858206600618No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT700.11043099640310469No Hit
GTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTGTAGAT690.10885341074020319No Hit
GTTTACCTCAGATCAGCCTGGGAAGGTGAGGGGAATGAAGCAGATGCTGT690.10885341074020319No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA680.1072758250773017No Hit
ACGCAGAGTACATGGGCAGGACCCCAGAACTGTGGAGTCAGTGGCAGCAT670.10569823941440021No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC670.10569823941440021No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG670.10569823941440021No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA660.1041206537514987No Hit
CCATTTAAATTCTGAAAAGGGATGGTGGATGTTTTCCCTCTCCTACGTTA660.1041206537514987No Hit
ATTTTTATTCTCTTGCTCTGAAAACTTGTATCAAAAAAAAAGAATTTAAA660.1041206537514987No Hit
GTTCTAAACACAGCTAGGACTCAGCAAGTCTAACACACTAAAATCATATG650.10254306808859721No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.10254306808859721No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC650.10254306808859721No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC650.10254306808859721No Hit
GCTTTAGGCACCAGCCTGCAACCCATTCGAGCAGCCACGTAGGCTGCACC650.10254306808859721No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC640.10096548242569572No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.10096548242569572No Hit
GAACAAAAGAAAGTCATAAAACTCCTGAATTTAATTTCCCCACCTAAGTC640.10096548242569572No Hit
GTACATGGGAGGGAGCCTCCTGCCCAGTCCCCCAACCCCAGCCGCTCGGA640.10096548242569572No Hit
GGTTTTGGGTGTAAAGAATGTTTACTGCCCTCCATTTAAATTCTGAAAAG640.10096548242569572No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA640.10096548242569572No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)