FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001420569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449590
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT51311.1412620387464134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21660.4817722814119531No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT17780.39547142952467806RNA PCR Primer, Index 39 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16360.3638870971329433No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG13990.31117240152138614RNA PCR Primer, Index 39 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT11830.2631286283057897RNA PCR Primer, Index 39 (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8480.18861629484641562No Hit
CTATTCACACTGAACACGTATGGCAGCTTAACCTACCCAAATATGAAGTT6390.14212949576280612No Hit
GTGTTACACTTATGCATTGCCAAAGTTTTTGTTAGTCTTGCATGCTTAAT5850.130118552458907No Hit
GTATTATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG5500.12233368179897239No Hit
CTACTACACAGGGGCCTAGCACCCTCCAGCTTCCAGCAGAGCGAAGGGAG5390.11988700816299294No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA5310.1181076091550079RNA PCR Primer, Index 39 (96% over 30bp)
GTTTAATTCTGAACCATTTTTTATAACCGCATTTTCTCTTGAAGCATCGT5200.11566093551902845No Hit
GTGTAACACAGTGACAAGAGAGCTTTTACAATTAAGTCTTCTAATACTGC4700.10453969171912186No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT4680.1040948419671256RNA PCR Primer, Index 39 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)