FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10060
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT226422.504970178926442No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT7267.216699801192843RNA PCR Primer, Index 23 (95% over 23bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG5975.934393638170974RNA PCR Primer, Index 23 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT5175.139165009940358RNA PCR Primer, Index 23 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGT2972.952286282306163No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT2262.246520874751491RNA PCR Primer, Index 23 (96% over 28bp)
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA2252.2365805168986084RNA PCR Primer, Index 23 (96% over 30bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1561.5506958250497018No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1541.5308151093439364No Hit
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG1291.2823061630218688Illumina PCR Primer Index 1 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTT970.9642147117296224No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGT900.8946322067594433No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.8548707753479126No Hit
ACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGC700.6958250497017893Illumina PCR Primer Index 1 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC660.6560636182902585No Hit
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC640.6361829025844931No Hit
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT610.6063618290258449RNA PCR Primer, Index 23 (95% over 22bp)
TCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCTGCTTG580.5765407554671969Illumina Single End Adapter 1 (96% over 25bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCT520.5168986083499005No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGACCTCGTATGCCGT470.46719681908548705No Hit
CTTTACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC450.44731610337972166RNA PCR Primer, Index 23 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTT420.41749502982107356No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAGAGAG370.36779324055666No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.35785288270377735Clontech SMART CDS Primer II A (100% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.31809145129224653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC300.2982107355864811No Hit
CTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCTGCTTGA280.2783300198807157Illumina Single End Adapter 1 (96% over 25bp)
CTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC280.2783300198807157No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATATCGTATGCCGT280.2783300198807157No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC280.2783300198807157No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAGAGA270.268389662027833No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTTCT250.2485089463220676No Hit
TCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTTCTGCTTG230.22862823061630216Illumina Single End Adapter 1 (100% over 25bp)
TCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGAA220.21868787276341947Illumina PCR Primer Index 1 (96% over 27bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC220.21868787276341947No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCTGCT220.21868787276341947Illumina Single End Adapter 1 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.20874751491053678No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC200.19880715705765406No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.19880715705765406No Hit
ATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTT180.17892644135188868Illumina PCR Primer Index 1 (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.17892644135188868No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGACCTCGTATGCCGTCTT180.17892644135188868No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.15904572564612326No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCAGAGAG150.14910536779324055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT150.14910536779324055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC130.12922465208747513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT130.12922465208747513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT120.11928429423459246No Hit
CTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTTCTGCTTGA120.11928429423459246Illumina Single End Adapter 1 (100% over 25bp)
CACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCTG120.11928429423459246RNA PCR Primer, Index 23 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC120.11928429423459246No Hit
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTC110.10934393638170974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)