FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19465
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGGAAGTTGGGTAGATGTGGGGACAACAGAGAGACTGTGGCAGAGG2301.1816080143847931No Hit
CTAATACCCATGCCGTGTTTTTTACCTTGGATTTAAAGTCACCTTAGGTT1590.8168507577703571No Hit
ATTCAAACTAATACCCATGCCGTGTTTTTTACCTTGGATTTAAAGTCACC1300.6678653994348831No Hit
AACTAATACCCATGCCGTGTTTTTTACCTTGGATTTAAAGTCACCTTAGG1230.6319034163883894No Hit
GTTTTTTACCTTGGATTTAAAGTCACCTTAGGTTGGGGCAACAGATTCTC1030.5291548933984074No Hit
TGTTTAAGATCTTGTTATTTCAGCTTCATAAGATCAAAGAGGAGTCTTTC920.47264320575391733No Hit
CCTTAGGTTGGGGCAACAGATTCTCACTCATGTTTAAGATCTTGTTATTT910.4675057796044182No Hit
CAGAGAGACTGTGGCAGAGGCAGGACTGCAGATCTATGGAAATTGCCTGG900.46236835345491906No Hit
CAAAGAGAGTTCTCATTTTCCCTATTCAAACTAATACCCATGCCGTGTTT820.42126894425892625No Hit
CTTTTACCCTCAGGATTCTCATCCCTTACAGCTGACTCTTCCAGGCAATT810.4161315181094272No Hit
GCTGTAAGGGATGAGAATCCTGAGGGTAAAAGAGAAAAGGGAAAGACTCC800.4109940919599281No Hit
GGGTAAAAGAGAAAAGGGAAAGACTCCTCTTTGATCTTATGAAGCTGAAA690.354482404315438No Hit
CATCAGAAGGGCAACAGTACAGGAAGTTGGGTAGATGTGGGGACAACAGA690.354482404315438No Hit
CTCAAAGAGAGTTCTCATTTTCCCTATTCAAACTAATACCCATGCCGTGT690.354482404315438No Hit
CTGCAGATCTATGGAAATTGCCTGGAAGAGTCAGCTGTAAGGGATGAGAA660.33907012586694063No Hit
GTCTTTCCCTTTTCTCTTTTACCCTCAGGATTCTCATCCCTTACAGCTGA650.33393269971744155No Hit
GTTTAAGATCTTGTTATTTCAGCTTCATAAGATCAAAGAGGAGTCTTTCC580.2979707166709479No Hit
GGGTAGATGTGGGGACAACAGAGAGACTGTGGCAGAGGCAGGACTGCAGA560.2876958643719496No Hit
CATTTTCCCTATTCAAACTAATACCCATGCCGTGTTTTTTACCTTGGATT560.2876958643719496No Hit
GAGTAACAGCTGCTTTTATTAACATCAGAAGGGCAACAGTACAGGAAGTT560.2876958643719496No Hit
GATTTAAAGTCACCTTAGGTTGGGGCAACAGATTCTCACTCATGTTTAAG510.26200873362445415No Hit
GATTCTCACTCATGTTTAAGATCTTGTTATTTCAGCTTCATAAGATCAAA510.26200873362445415No Hit
GATCAAAGAGGAGTCTTTCCCTTTTCTCTTTTACCCTCAGGATTCTCATC500.25687130747495507No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.251733881325456No Hit
GCTTTTATTAACATCAGAAGGGCAACAGTACAGGAAGTTGGGTAGATGTG490.251733881325456No Hit
GATGTGGGGACAACAGAGAGACTGTGGCAGAGGCAGGACTGCAGATCTAT480.24659645517595682No Hit
TCTTTGATCTTATGAAGCTGAAATAACAAGATCTTAAACATGAGTGAGAA470.24145902902645774No Hit
TACCCATGCCGTGTTTTTTACCTTGGATTTAAAGTCACCTTAGGTTGGGG460.23632160287695866No Hit
ACAGAGAGACTGTGGCAGAGGCAGGACTGCAGATCTATGGAAATTGCCTG450.23118417672745953No Hit
ATCTTAAACATGAGTGAGAATCTGTTGCCCCAACCTAAGGTGACTTTAAA430.22090932442846134No Hit
GATCTATGGAAATTGCCTGGAAGAGTCAGCTGTAAGGGATGAGAATCCTG430.22090932442846134No Hit
GCCTGGAAGAGTCAGCTGTAAGGGATGAGAATCCTGAGGGTAAAAGAGAA430.22090932442846134No Hit
GGGAAAGACTCCTCTTTGATCTTATGAAGCTGAAATAACAAGATCTTAAA420.21577189827896226No Hit
TCCCTATTCAAACTAATACCCATGCCGTGTTTTTTACCTTGGATTTAAAG400.20549704597996404No Hit
TTCTCATCCCTTACAGCTGACTCTTCCAGGCAATTTCCATAGATCTGCAG390.20035961983046494No Hit
GGGAGTAACAGCTGCTTTTATTAACATCAGAAGGGCAACAGTACAGGAAG390.20035961983046494No Hit
GACTGTGGCAGAGGCAGGACTGCAGATCTATGGAAATTGCCTGGAAGAGT380.19522219368096583No Hit
GCTCAAAGAGAGTTCTCATTTTCCCTATTCAAACTAATACCCATGCCGTG360.18494734138196764No Hit
AGGCAGGACTGCAGATCTATGGAAATTGCCTGGAAGAGTCAGCTGTAAGG360.18494734138196764No Hit
GGTTGGGGCAACAGATTCTCACTCATGTTTAAGATCTTGTTATTTCAGCT360.18494734138196764No Hit
GGGCAACAGATTCTCACTCATGTTTAAGATCTTGTTATTTCAGCTTCATA360.18494734138196764No Hit
CCCATGCCGTGTTTTTTACCTTGGATTTAAAGTCACCTTAGGTTGGGGCA340.17467248908296942No Hit
TGTTATTTCAGCTTCATAAGATCAAAGAGGAGTCTTTCCCTTTTCTCTTT340.17467248908296942No Hit
GGACTGCAGATCTATGGAAATTGCCTGGAAGAGTCAGCTGTAAGGGATGA330.16953506293347032No Hit
GGGCAACAGTACAGGAAGTTGGGTAGATGTGGGGACAACAGAGAGACTGT330.16953506293347032No Hit
GTTCTCATTTTCCCTATTCAAACTAATACCCATGCCGTGTTTTTTACCTT320.16439763678397123No Hit
CAACAGAGAGACTGTGGCAGAGGCAGGACTGCAGATCTATGGAAATTGCC320.16439763678397123No Hit
CAGTACAGGAAGTTGGGTAGATGTGGGGACAACAGAGAGACTGTGGCAGA320.16439763678397123No Hit
GGATGAGAATCCTGAGGGTAAAAGAGAAAAGGGAAAGACTCCTCTTTGAT320.16439763678397123No Hit
CCTTACAGCTGACTCTTCCAGGCAATTTCCATAGATCTGCAGTCCTGCCT310.15926021063447213No Hit
TCTTAAACATGAGTGAGAATCTGTTGCCCCAACCTAAGGTGACTTTAAAT300.15412278448497305No Hit
GAAATAACAAGATCTTAAACATGAGTGAGAATCTGTTGCCCCAACCTAAG290.14898535833547394No Hit
GATCTGCAGTCCTGCCTCTGCCACAGTCTCTCTGTTGTCCCCACATCTAC290.14898535833547394No Hit
CAACAGTACAGGAAGTTGGGTAGATGTGGGGACAACAGAGAGACTGTGGC280.1438479321859748No Hit
TCATAAGATCAAAGAGGAGTCTTTCCCTTTTCTCTTTTACCCTCAGGATT270.13871050603647572No Hit
GGATTTAAAGTCACCTTAGGTTGGGGCAACAGATTCTCACTCATGTTTAA270.13871050603647572No Hit
GTCTCTCTGTTGTCCCCACATCTACCCAACTTCCTGTACTGTTGCCCTTC260.13357307988697661No Hit
CAGGATTCTCATCCCTTACAGCTGACTCTTCCAGGCAATTTCCATAGATC260.13357307988697661No Hit
TTCTCACTCATGTTTAAGATCTTGTTATTTCAGCTTCATAAGATCAAAGA240.12329822758797841No Hit
GGCAGAGGCAGGACTGCAGATCTATGGAAATTGCCTGGAAGAGTCAGCTG240.12329822758797841No Hit
ACCTTAGGTTGGGGCAACAGATTCTCACTCATGTTTAAGATCTTGTTATT240.12329822758797841No Hit
AAAGAGGAGTCTTTCCCTTTTCTCTTTTACCCTCAGGATTCTCATCCCTT230.11816080143847933No Hit
ATCTTGTTATTTCAGCTTCATAAGATCAAAGAGGAGTCTTTCCCTTTTCT230.11816080143847933No Hit
AACCTAAGGTGACTTTAAATCCAAGGTAAAAAACACGGCATGGGTATTAG230.11816080143847933No Hit
AGAATCCTGAGGGTAAAAGAGAAAAGGGAAAGACTCCTCTTTGATCTTAT220.11302337528898021No Hit
GTAAAAAACACGGCATGGGTATTAGTTTGAATAGGGAAAATGAGAACTCT220.11302337528898021No Hit
TTTATTAACATCAGAAGGGCAACAGTACAGGAAGTTGGGTAGATGTGGGG220.11302337528898021No Hit
CACTCATGTTTAAGATCTTGTTATTTCAGCTTCATAAGATCAAAGAGGAG220.11302337528898021No Hit
GAGTTCTCATTTTCCCTATTCAAACTAATACCCATGCCGTGTTTTTTACC220.11302337528898021No Hit
AAGTTGGGTAGATGTGGGGACAACAGAGAGACTGTGGCAGAGGCAGGACT210.10788594913948113No Hit
GTCCTGCCTCTGCCACAGTCTCTCTGTTGTCCCCACATCTACCCAACTTC210.10788594913948113No Hit
GAAGGGCAACAGTACAGGAAGTTGGGTAGATGTGGGGACAACAGAGAGAC210.10788594913948113No Hit
AGTCAGCTGTAAGGGATGAGAATCCTGAGGGTAAAAGAGAAAAGGGAAAG210.10788594913948113No Hit
ACCTTGGATTTAAAGTCACCTTAGGTTGGGGCAACAGATTCTCACTCATG210.10788594913948113No Hit
AGGTTGGGGCAACAGATTCTCACTCATGTTTAAGATCTTGTTATTTCAGC210.10788594913948113No Hit
CCCTTTTCTCTTTTACCCTCAGGATTCTCATCCCTTACAGCTGACTCTTC210.10788594913948113No Hit
CATAAGATCAAAGAGGAGTCTTTCCCTTTTCTCTTTTACCCTCAGGATTC200.10274852298998202No Hit
CCCTTACAGCTGACTCTTCCAGGCAATTTCCATAGATCTGCAGTCCTGCC200.10274852298998202No Hit
TAACAGCTGCTTTTATTAACATCAGAAGGGCAACAGTACAGGAAGTTGGG200.10274852298998202No Hit
TGAGAATCCTGAGGGTAAAAGAGAAAAGGGAAAGACTCCTCTTTGATCTT200.10274852298998202No Hit
CTATGGAAATTGCCTGGAAGAGTCAGCTGTAAGGGATGAGAATCCTGAGG200.10274852298998202No Hit
CAGCTGCTTTTATTAACATCAGAAGGGCAACAGTACAGGAAGTTGGGTAG200.10274852298998202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)