FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001420585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382832
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT29560.7721402599573703No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10980.28680988005182434No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT10720.2800183892673549RNA PCR Primer, Index 17 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9040.23613491035232165No Hit
GTATAGACAAGCTTTGTAGTGAAGTATAGTAGCAATAATTTCTGTACCTG7280.190161741965144No Hit
GTACATGAGAATCCTTTTTTGACAAGCCTTGGAAAGCTGACACTGTCTCT6940.1812805617085301No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG6930.18101935052451207RNA PCR Primer, Index 17 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT6790.17736239394825928RNA PCR Primer, Index 17 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4650.12146320056839555No Hit
GTATACAGTGATGTATTTACTAAACAGAGACCTGTGCAGAAATTACATAC4610.12041835583232331No Hit
GTTTTAAGAATTCCCCGTAGGAATTATCTCCCAGGTCCCCCGCTAGAATT4150.1084026413674928No Hit
TCACCAGACTGTCCATTAATTCACCTCTGAAAAAGTGGCATTTAATTTCA4120.10761900781543862No Hit
TGTTAATACTAACCCTTAAAAAAAAGAAATAGGAAAAGAAAGGCTGCAAT4120.10761900781543862No Hit
CTACTACACAGGGGCCTAGCACCCTCCAGCTTCCAGCAGAGCGAAGGGAG4070.10631295189534835No Hit
TGTCTATACAGTATCATATGACCTTTGATTATGAAAAAATCAGAATAAAA4060.10605174071133029No Hit
ACATAGTAGAGGCTGGAAAGAGCCCCCAAACCTGTGCCCATGCCCCTCCA3860.1008275170309692No Hit
GTGTATGGGCAGAAAAGCAGATTTGTGTCCTTCAGAAGGGAAATGTAAAA3860.1008275170309692No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)