FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001420586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382832
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGAGAATCCTTTTTTGACAAGCCTTGGAAAGCTGACACTGTCTCT7170.1872884189409454No Hit
GTATAGACAAGCTTTGTAGTGAAGTATAGTAGCAATAATTTCTGTACCTG6340.16560789066744683No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC5240.1368746604254608No Hit
GTATACAGTGATGTATTTACTAAACAGAGACCTGTGCAGAAATTACATAC5010.1308668031930455No Hit
TGTTAATACTAACCCTTAAAAAAAAGAAATAGGAAAAGAAAGGCTGCAAT4560.11911229991223304No Hit
TCACCAGACTGTCCATTAATTCACCTCTGAAAAAGTGGCATTTAATTTCA4330.11310444267981778No Hit
GTGTATGGGCAGAAAAGCAGATTTGTGTCCTTCAGAAGGGAAATGTAAAA4170.10892506373552889No Hit
GTTTTAAGAATTCCCCGTAGGAATTATCTCCCAGGTCCCCCGCTAGAATT4110.10735779663142057No Hit
GTATTAACAAATGGCAATGAGTAGAAAAGTTAACATGAAGATGAAGAAGA3960.10343962887114976No Hit
GTCTTACTCTGTCACCCAGGCTGGAGTGCAGTAGTGCGATCTCAGCTCAC3870.10108872821498725No Hit
GTATAACATCACTGTCAAACTTTGTCACCCTAACTTCGTATTTTTTGATA3850.10056630584695116No Hit
CTACTACACAGGGGCCTAGCACCCTCCAGCTTCCAGCAGAGCGAAGGGAG3840.10030509466293308No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)