FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001420595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT216610.541525No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129930.324825No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT98340.24585RNA PCR Primer, Index 31 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93960.2349No Hit
TTCTTACACCGCGAGGAAGCTTCCTCGCAACTTTGTGGTAGATTACTATG71640.1791No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69190.172975No Hit
GTCCAGGAGTACGTGTATGACCTGGAACTGAACTGAGCTGCTCAGAGACA60700.15175No Hit
CAATAATACAGCACATAAAAGAGATTACATTAAGAGAAGAGACAGGAACT53830.134575No Hit
TCCCTGAAGACTTCCTGTCTCTGAGCAGCTCAGTTCAGTTCCAGGTCATA53130.132825No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT52690.131725RNA PCR Primer, Index 31 (95% over 24bp)
TCTCTGAGCAGCTCAGTTCAGTTCCAGGTCATACACGTACTCCTGGACCC50230.125575No Hit
CTCCTGGACCCAGGACTCACTGGGGTCAGCGCAGACTTGCTTGCCTCTTT48330.12082499999999999No Hit
CTCCTGGACCCAGGATTCACTGGGATCAGCACAGACTTGCTTGCTTCTTT42390.10597499999999999No Hit
CAATAACACGGCACATAAAAAAGATTAAATTAAGAGAAGGGACAGGAACT41170.102925No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)