FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001420597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422505
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT146633.4704914734736865No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT69351.6414006934829175TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT41630.9853137832688371RNA PCR Primer, Index 10 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17050.40354552017135886No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15820.37443343865753065No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT12430.2941977018023455TruSeq Adapter, Index 10 (96% over 26bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG12380.29301428385462897TruSeq Adapter, Index 10 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG11280.26697908900486383TruSeq Adapter, Index 10 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10220.241890628513272No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC7580.17940616087383582No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7290.17254233677707956Clontech SMART CDS Primer II A (100% over 21bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT7260.1718322860084496No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)