FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108366
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT1331012.282450215012089No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT65816.072938006385767RNA PCR Primer, Index 46 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT39303.626598748685012RNA PCR Primer, Index 46 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38353.5389328756251963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28312.612443017182511No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22702.0947529667977043No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG8950.8259048040898437TruSeq Adapter, Index 4 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC8840.8157540187881809No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7670.7077865751250392Clontech SMART CDS Primer II A (100% over 21bp)
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT7100.6551870512891497No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6810.6284258900393113No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6690.6173523060738608No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT5300.4890832918073935RNA PCR Primer, Index 46 (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.4863148958160308No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG5240.48354649982466824RNA PCR Primer, Index 46 (96% over 28bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGCG3520.3248251296532123No Hit
GTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT3270.3017551630585239No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.26115202185187236No Hit
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC2760.2546924312053596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2700.2491556392226344No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC2310.21316649133492055No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC2280.21039809534355794TruSeq Adapter, Index 18 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2020.18640533008508203No Hit
CTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC1910.17625454478341915No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.17071775280069396No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA1710.15779857150766846RNA PCR Primer, Index 46 (96% over 28bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1500.13841979956813022Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1410.13011461159404242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1370.12642341693889228No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT1300.11996382629237952No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.11534983297344185No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1240.11442703430965431Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.11165863831829172No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT1200.11073583965450418No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGT1160.10704464499935404No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1150.10612184633556651No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)