FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96557
Sequences flagged as poor quality0
Sequence length100
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT1845819.116169723582964No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT87069.016435887610427RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT52135.398883561005417RNA PCR Primer, Index 26 (100% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48675.040545998736498No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34583.5813043073003508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32703.3866006607496093No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14851.5379516762119785No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG14191.4695982683803348RNA PCR Primer, Index 26 (100% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13771.4261006452147436No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG13491.3971022297710163RNA PCR Primer, Index 26 (96% over 27bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12731.3183922449951841No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT12321.2759302795240117RNA PCR Primer, Index 26 (100% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC11411.1816854293318972RNA PCR Primer, Index 26 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT8750.9062004826164856RNA PCR Primer, Index 26 (100% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7380.7643153784811044Clontech SMART CDS Primer II A (100% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5890.6100023820126972No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC5100.5281854241536088RNA PCR Primer, Index 26 (96% over 28bp)
GTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT5080.526114108764771No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.4670816201828971No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA4240.4391188624335885RNA PCR Primer, Index 26 (100% over 29bp)
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC3960.41012044698986094No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGAG3900.4039065008233479No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC3210.33244611990844786No Hit
CTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC2540.2630570543823855RNA PCR Primer, Index 26 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2140.22163074660563192No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2050.21230982735586235Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.19988193502283624No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGT1870.19366798885632322No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.1802044388288783No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGT1710.17709746574562174No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.1708835195791087No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1380.1429207618298No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1360.14084944644096234Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGATGCATCAGACCTACAGCAGACATTGCAGGCCTGAAGAAA1350.1398137887465435No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT1270.13152852719119276No Hit
GGGTACAACAGAGCACTTTACATCCAAAGGCAGAGAGGGACACAGCAATG1220.12635023871909856No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGT1210.1253145810246797No Hit
GTGTGGGAGATGCTGTTTCTGCCATGTGAGAATGTGATGGCAGAATTAAC1150.11910063485816669No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1130.11702931946932899Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGTACCCCTGGTGGGGTTGTGTTAATTCTGCCATCACATTCTCACATG1110.11495800408049131No Hit
GTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT1110.11495800408049131RNA PCR Primer, Index 26 (100% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1080.1118510309972348No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1060.10977971560839712No Hit
GTACAGAGCTGGTGATATTCATTCCTAGACTACCTCGGACCAGGCCTTTT1060.10977971560839712No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGA1050.10874405791397826No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG1020.10563708483072175TruSeq Adapter, Index 1 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1020.10563708483072175No Hit
GCTCTGTACTGGCTGCATCAGGGATCTTAATCAACACATTTAGCTTGGCG1000.10356576944188407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)