FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96557
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61716.391043632258666No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13881.4374928798533508No Hit
CATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTCGCCGT3000.31069730832565223Illumina RNA PCR Primer (100% over 25bp)
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG2540.2630570543823855No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.24959350435494063No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2000.20713153888376815No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.1853827273009725No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1610.16674088880143334No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1580.16363391571817684Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1530.15845562724608261Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1520.1574199695516638No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCC1340.13877813105212466No Hit
ATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTC1330.1377424733577058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.1356711579688681No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC1270.13152852719119276No Hit
GTACATGGGGATGCATCAGACCTACAGCAGACATTGCAGGCCTGAAGAAA1250.1294572118023551No Hit
GTACAGAGCTGGTGATATTCATTCCTAGACTACCTCGGACCAGGCCTTTT1220.12635023871909856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC1190.12324326563584204No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1170.12117195024700436No Hit
GTTGTACCCCTGGTGGGGTTGTGTTAATTCTGCCATCACATTCTCACATG1090.11288668869165364No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1090.11288668869165364Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTCTGTACTGGCTGCATCAGGGATCTTAATCAACACATTTAGCTTGGCG1040.10770840021955942No Hit
ATACACACACAGTTCATAATTGGATTACATATAATAATAATATCAACAAG1030.10667274252514058No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCC1010.10460142713630291No Hit
GGGTACAACAGAGCACTTTACATCCAAAGGCAGAGAGGGACACAGCAATG1000.10356576944188407No Hit
GTGTGGGAGATGCTGTTTCTGCCATGTGAGAATGTGATGGCAGAATTAAC990.10253011174746524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)