FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14132
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT481034.03622983300311No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT165511.711010472686102RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT10817.6493065383526755RNA PCR Primer, Index 27 (96% over 25bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG9786.920464194735352RNA PCR Primer, Index 27 (96% over 30bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT4883.453155958109256RNA PCR Primer, Index 27 (96% over 28bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA3312.3422020945372206RNA PCR Primer, Index 27 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGT2821.9954712708746107No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG2461.7407302575714692RNA PCR Primer, Index 27 (100% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC1651.1675629776393999No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC1511.0684970280215116No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT1370.969431078403623RNA PCR Primer, Index 27 (95% over 22bp)
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGC1360.9623549391452024TruSeq Adapter, Index 27 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGT1020.7217662043589017No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTT880.6227002547410133No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.5448627228983867No Hit
CTTTACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC760.537786583639966RNA PCR Primer, Index 27 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC690.4882536088310218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.4882536088310218No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTACCTCGTATGCCGT610.431644494763657No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTTCT590.4174922162468157RNA PCR Primer, Index 38 (95% over 21bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGGACTCC560.3962637984715539No Hit
ATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTT400.2830455703368242RNA PCR Primer, Index 27 (100% over 27bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACGGACTCC310.21936031701103878No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATATCGTATGCCGT290.20520803849419758No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.20520803849419758No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGGACTC270.19105575997735635No Hit
TCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTTCTGCTTG240.16982734220209456RNA PCR Primer, Index 38 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCCTCT220.15567506368525333RNA PCR Primer, Index 27 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGTCTT210.14859892442683273No Hit
CATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTTCTGCT200.1415227851684121RNA PCR Primer, Index 38 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCCT180.1273705066515709RNA PCR Primer, Index 27 (95% over 21bp)
CTTATCACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC180.1273705066515709No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC180.1273705066515709No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTACCTCGTATGCCGTCTT170.1202943673931503No Hit
CTTAACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC170.1202943673931503RNA PCR Primer, Index 27 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGTCTTCT150.10614208887630909RNA PCR Primer, Index 38 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC150.10614208887630909No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)