FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001420685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165578
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT27131.6385026996340095No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14810.8944424983995459No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13100.7911679087801519No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT9660.5834108396042953RNA PCR Primer, Index 23 (95% over 23bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG6930.4185338631943857RNA PCR Primer, Index 23 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6800.4106825786034376No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT6110.3690103757745594RNA PCR Primer, Index 23 (96% over 25bp)
GTACATGGGAGGGCACATTCGCCTCCCAGGTGATGCTGGAGGGGGACAAG2820.17031248112671973No Hit
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA2820.17031248112671973RNA PCR Primer, Index 23 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGT2820.17031248112671973No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT2720.16427303144137506RNA PCR Primer, Index 23 (96% over 28bp)
GTACATTGTAGAGCCGAACTTTGAGTTACTGTGCAAGATTTTTTTTTCAT2570.15521385691335807No Hit
GTTAATAACAGTGGTTTGACTAATTGTATTGGCTGTGTGGATGTGGAACA2320.14011523269999637No Hit
CTACTACACAGGGGCCTAGCACCCTCCAGCTTCCAGCAGAGCGAAGGGAG2210.13347183804611726No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.12380871854956577Clontech SMART CDS Primer II A (100% over 21bp)
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG2050.12380871854956577No Hit
GTAATATGTTTTGTGAGAATCCTTGGGATTAAAGTTTTGGTTACAAATTG2030.12260082861249684No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC2000.12078899370689343No Hit
CTTCCAGAGTCATCTACCTGAGTCCCAAAGCTCCCTGTCCTGAAAGCCAC1920.11595743395861768No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.11172981917887642No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA1790.10810614936766963No Hit
CTCTGGGAGGGGCTCAAATCCGCCCAATCTCCTTCAGCTTGGCCACCAGG1760.10629431446206622No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA1750.10569036949353175No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG1730.10448247955646282No Hit
ATAATATCCCGGGGCAAACCGGAGAATTTGGCAGTCCGATTGGGGGGGGG1720.10387853458792835No Hit
GTACATGGGATTCTTCCACAAGAGAGGGCTTTCTCAGGACCTGGTTGCTA1690.10206669968232496No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)