FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001420686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165578
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4170.2518450518788728No Hit
GTACATGGGAGGGCACATTCGCCTCCCAGGTGATGCTGGAGGGGGACAAG3290.19869789464783968No Hit
GTACATTGTAGAGCCGAACTTTGAGTTACTGTGCAAGATTTTTTTTTCAT2840.17152037106378867No Hit
GTTAATAACAGTGGTTTGACTAATTGTATTGGCTGTGTGGATGTGGAACA2230.13467972798318617No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG2200.1328678930775828No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC2160.1304521132034449No Hit
CTACTACACAGGGGCCTAGCACCCTCCAGCTTCCAGCAGAGCGAAGGGAG2150.12984816823491044No Hit
CTTCCAGAGTCATCTACCTGAGTCCCAAAGCTCCCTGTCCTGAAAGCCAC1960.11837321383275556No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG1840.11112587421034195No Hit
CTCTAATTCTCTTCTCAAATCCCAAGGCAAAACAATGGAGACTTAATGTG1830.1105219292418075No Hit
GTTTTAAGAAAATGGCAGACAAACCAGACATGGGGGAAATCGCCAGCTTC1820.10991798427327303No Hit
GTAATATGTTTTGTGAGAATCCTTGGGATTAAAGTTTTGGTTACAAATTG1800.10871009433620407No Hit
GTATATGGGACAGGGGAGGTGATAGCATTGCTTTCGTGTCCTTAATACTT1780.10750220439913515No Hit
GTATTGGCTGTGTGGATGTGGAACAGAAGATTCTTGATGTCAAGAACGGC1770.10689825943060068No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA1770.10689825943060068No Hit
GAATTAGAGAGGTGAGGATCTGGTATTTCCTGGACTAAATTCCCCTTGGG1720.10387853458792835No Hit
CCTTAACATTTGGGGGATTTTGATTCCCACAAAAACCTCTATCATTTAAA1710.10327458961939388No Hit
GAACGAGACTGCACGGATTGTTTTAAGAAAATGGCAGACAAACCAGACAT1680.10146275471379047No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)