FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001420689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511831
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36940.7217225998425262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30300.5919922787013682No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT22720.44389652053119094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15900.3106494135759655No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT8710.17017335800293457TruSeq Adapter, Index 10 (95% over 21bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG6700.13090258307918043TruSeq Adapter, Index 10 (96% over 28bp)
ATTTTATACTATGGTGGCAAAAAAGAAGACTTCAAGCGTTTGGGGGAAAA6330.12367363446137494No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT6290.12289212650269327RNA PCR Primer, Index 10 (95% over 23bp)
GTATACGGAGGGCACGACTGCTGGCTTCCAAAGACTCTGTGAACTAGATC5320.10394055850466268No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)