FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001420691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences942468
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80840.857747955368246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63700.6758850167857158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32790.34791632182737237No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT30570.3243611454181999No Hit
GTCTAAGATTGGTCATACATTCCCAATTTACTCTCAGTTCCAGTCAAGCT20050.2127393184702292No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11780.12499098112614963No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT11610.12318720635607788RNA PCR Primer, Index 33 (95% over 22bp)
CTTTATTAGTGATGGTAGCATACATATTAGAGAAGGTAGCTAAAGGCAAG11280.11968576121417385No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10450.11087909616029404Clontech SMART CDS Primer II A (100% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9430.10005644753986342No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)