FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14618
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT389426.63839102476399No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT177412.13572308113285RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT12298.407442878642769RNA PCR Primer, Index 26 (100% over 24bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG11657.96962648789164RNA PCR Primer, Index 26 (100% over 29bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT4943.3793952661102753RNA PCR Primer, Index 26 (100% over 27bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA4112.8116021343549047RNA PCR Primer, Index 26 (100% over 29bp)
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG3092.1138322615952934RNA PCR Primer, Index 26 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGT2441.669174989738678No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC1551.06033657135039RNA PCR Primer, Index 26 (95% over 22bp)
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC1491.0192912847174715RNA PCR Primer, Index 26 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT1440.9850868791900397RNA PCR Primer, Index 26 (100% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC1310.8961554248187167No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGT1270.868791900396771No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTT1150.7867013271309344RNA PCR Primer, Index 26 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC910.6225201805992612No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGAG660.4514981529621015No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCT650.44465727185661513RNA PCR Primer, Index 26 (95% over 24bp)
CTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC570.38993022301272406RNA PCR Primer, Index 26 (95% over 22bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACTCCTGAG530.36256669859077845No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGT530.36256669859077845No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTT490.33520317416883294RNA PCR Primer, Index 26 (95% over 22bp)
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT450.3078396497468874RNA PCR Primer, Index 26 (96% over 30bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.28731700643042823No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTTCT370.2531126009029963RNA PCR Primer, Index 26 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.2531126009029963No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATATCGTATGCCGT320.21890819537556439No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGA300.20522643316459158No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGTCTT290.1983855520591052RNA PCR Primer, Index 26 (95% over 22bp)
TCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCTGCTTG250.17102202763715968RNA PCR Primer, Index 26 (96% over 29bp)
TCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTTCTGCTTG250.17102202763715968RNA PCR Primer, Index 26 (96% over 29bp)
TCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAA240.1641811465316733RNA PCR Primer, Index 26 (96% over 31bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.1504993843207005No Hit
CTTATCACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC190.12997674100424134RNA PCR Primer, Index 26 (95% over 22bp)
CATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCTGCT180.12313585989875496RNA PCR Primer, Index 26 (96% over 27bp)
CACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCTG170.11629497879326858TruSeq Adapter, Index 3 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC170.11629497879326858No Hit
CTTAACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC150.10261321658229579No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGG150.10261321658229579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)