FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001420707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525518
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT46870.89188191460616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22680.4315741801422596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20250.3853340894127318No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT18270.34765697844793136RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT11100.2112201675299419RNA PCR Primer, Index 39 (96% over 25bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG11030.20988814845542872RNA PCR Primer, Index 39 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10260.19523593863578412No Hit
GTTTTAAGAATTCCCCGTAGGAATTATCTCCCAGGTCCCCCGCTAGAATT8080.1537530588866604No Hit
GTATAAACAGTAACCATTCCACTTATCACAGCTTGGTTGAGTTTAAAATT6230.1185496976316701No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)