FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001420709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684745
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT201232.938758223864358No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT77901.1376497820356484RNA PCR Primer, Index 31 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50230.733557747774719No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45260.6609759837603779No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT42140.6154115765723006RNA PCR Primer, Index 31 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25260.3688964505034721No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14410.21044330371160067Clontech SMART CDS Primer II A (100% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC9800.14311897129588386No Hit
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT8630.12603231860035488RNA PCR Primer, Index 31 (95% over 21bp)
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG7610.11113626240425267RNA PCR Primer, Index 38 (95% over 24bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)