FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67909
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT1755625.85224344343165No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT810511.935089605206969RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT49657.311254767409327RNA PCR Primer, Index 33 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15302.2530150642771947No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG11951.759707844321077RNA PCR Primer, Index 15 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11161.6433756939433652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11031.624232428691337No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC10501.5461868088176824No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT8221.2104433874744143RNA PCR Primer, Index 33 (95% over 21bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT6070.8938432313831745RNA PCR Primer, Index 33 (96% over 27bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG5680.8364134356270891RNA PCR Primer, Index 33 (96% over 29bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC4810.7083008143250527No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4470.6582338128966705Clontech SMART CDS Primer II A (100% over 21bp)
GTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT4430.652343577434508No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.5360114270567966No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC3500.5153956029392275No Hit
CTTTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC3230.4756365135696299No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.43587742420003234No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2670.39317321709935354No Hit
CTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC2600.382865305040569No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC2540.37402995184732507RNA PCR Primer, Index 15 (96% over 25bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.2783136255871828No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGT1770.26064291920069504No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA1700.25033500714191054RNA PCR Primer, Index 33 (96% over 29bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG1580.2326643007554227TruSeq Adapter, Index 1 (96% over 28bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG1470.21646615323447554RNA PCR Primer, Index 17 (96% over 28bp)
GTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT1350.19879544684798775RNA PCR Primer, Index 33 (95% over 22bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG1270.18701497592366254RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG1260.18554241705812188RNA PCR Primer, Index 31 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAACCTCGTATGCCGT1210.17817962273041865No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGT1150.16934426953717474No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG1130.16639915180609344TruSeq Adapter, Index 3 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGT1130.16639915180609344No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG1120.1649265929405528RNA PCR Primer, Index 46 (96% over 28bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGG1060.1560912397473089No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG950.13989309222636176RNA PCR Primer, Index 26 (100% over 29bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG800.117804709243252Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG800.117804709243252RNA PCR Primer, Index 39 (96% over 30bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.10896935605000808No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTT740.10896935605000808No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA720.1060242383189268RNA PCR Primer, Index 1 (96% over 28bp)
CTTATAACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG720.1060242383189268No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT710.10455167945338614Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT700.1030791205878455Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.1030791205878455No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCATCTCGTATGCCGTC700.1030791205878455No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA690.10160656172230485Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)