FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67909
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18472.7198162246535804No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4180.6155296057959917No Hit
CATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTCGCCGT3500.5153956029392275Illumina RNA PCR Primer (100% over 25bp)
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG3150.4638560426453047No Hit
ATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTC1980.2915666553770487No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA760.1119144737810894Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)