FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47068
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT1289527.396532676128153No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT630813.401886632106738TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT44309.411914676638055RNA PCR Primer, Index 11 (95% over 23bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG18844.002719469703408TruSeq Adapter, Index 11 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT13632.8958103169881872TruSeq Adapter, Index 11 (96% over 26bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG11352.4114047760686668TruSeq Adapter, Index 11 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6971.480836236933798No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC6961.4787116512280105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6771.4383445228180505No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT6391.3576102659981304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5651.200390923769865No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC5381.1430271097136058RNA PCR Primer, Index 17 (95% over 24bp)
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA5211.1069091527152206RNA PCR Primer, Index 11 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.5736381405625903No Hit
CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC2420.5141497408005439No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACTA2270.4822809552137333No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC1960.41641879833432477No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.3845500127475142Clontech SMART CDS Primer II A (100% over 21bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG1640.34843205574912894TruSeq Adapter, Index 3 (96% over 28bp)
CTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC1530.3250616129854678No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.2910682416928699No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGT1350.28681907028129516No Hit
ATTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT1310.2783207274581457No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG1110.23582901334239822RNA PCR Primer, Index 33 (96% over 29bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG1010.2145831562845245TruSeq Adapter, Index 1 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.21033398487294977No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG940.1997110563440129RNA PCR Primer, Index 23 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGT900.19121271352086341No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCGTACTA860.18271437069771393No Hit
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTT850.18058978499192657TruSeq Adapter, Index 11 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGT800.16996685646298973No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGTCTT770.1635930993456276No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGT770.1635930993456276No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG750.15934392793405286RNA PCR Primer, Index 31 (96% over 29bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.1572193422282655No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG700.148720999405116TruSeq Adapter, Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTT690.14659641369932863No Hit
CCTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT660.1402226565819665No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.12959972805302966No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG600.12747514234724228RNA PCR Primer, Index 39 (96% over 30bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACT600.12747514234724228No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG580.12322597093566755RNA PCR Primer, Index 17 (96% over 28bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG560.1189767995240928Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTCT560.1189767995240928Illumina PCR Primer Index 9 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGTCTT530.11260304240673069No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG510.10835387099515593RNA PCR Primer, Index 27 (96% over 30bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.10410469958358119No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.10410469958358119No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)