FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123468
Sequences flagged as poor quality0
Sequence length100
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT2469520.00113389704215No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT116029.396766773576958TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT71365.779635209123011RNA PCR Primer, Index 1 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58674.751838533061198No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49624.018855087958014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41453.3571451712184537No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26402.1382058509087374No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19051.5429099037807366No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18731.516992257103055No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG13261.073962484206434TruSeq Adapter, Index 1 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC13011.0537143227394952No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT11670.9451841772767033No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10200.8261249878511031No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG9700.7856286649172255TruSeq Adapter, Index 1 (96% over 28bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8800.7127352836362458No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8660.70139631321476Clontech SMART CDS Primer II A (100% over 21bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT7990.6471312404833641TruSeq Adapter, Index 1 (96% over 26bp)
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC4620.37418602390902905No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC4360.3531279359834127No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4110.3328797745164739No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC3640.294813230958629Illumina PCR Primer Index 5 (95% over 24bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAGGCAT3610.29238345158259627No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3520.2850941134544983No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3340.2705154371983024No Hit
CTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC3060.24783749635533095No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG2930.23730845239252277TruSeq Adapter, Index 3 (96% over 28bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG2620.21220073217351867RNA PCR Primer, Index 33 (96% over 29bp)
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA2490.2016716882107105RNA PCR Primer, Index 1 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATATCGTATGCCGT2060.16684485048757572No Hit
ATTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT1990.16117536527683285No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1970.15955551235947776No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGAACTCGTATGCCGT1930.15631580652476756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1750.1417371302685716No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1710.1384974244338614Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1700.13768749797518387Clontech Universal Primer Mix Long (96% over 26bp)
GCCTTAGAGAGGGCAGTTTTATTGTCTCAGAGGGGCAGGGCTGAGGGAGG1620.13120808630576344No Hit
ATATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.1271584540123757No Hit
AGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.12391874817766547No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG1490.12067904234295526RNA PCR Primer, Index 31 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1450.11743933650824505No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT1430.11581948359088994No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG1380.11176985129750219RNA PCR Primer, Index 26 (100% over 29bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG1370.11095992483882464RNA PCR Primer, Index 17 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGACCTCGTATGCCGT1340.10853014546279198No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGAGCTCGTATGCCGT1300.10529043962808178No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG1270.10286066025204912RNA PCR Primer, Index 27 (96% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)