FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123468
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83516.763695856416238No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23131.873359898921178No Hit
CATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTCGCCGT11680.9459941037353808Illumina Single End PCR Primer 1 (100% over 23bp)
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG9840.7969676353387113No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC6250.50620403667347No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2750.2227297761363268No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750.2227297761363268No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC2090.1692746298636084No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2080.16846470340493083No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2070.16765477694625328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.15955551235947776No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1700.13768749797518387Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC1630.13201801276444097No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1620.13120808630576344Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCC1440.11662941004956749No Hit
GCCTTAGAGAGGGCAGTTTTATTGTCTCAGAGGGGCAGGGCTGAGGGAGG1360.11014999838014708No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1320.10691029254543687No Hit
AAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1310.10610036608675934No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC1290.10448051316940421No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.10367058671072665No Hit
GTACATGGGGCTCCCAACCAAGCCCTCCAGCAAGGATTCAGAGTGCCCCT1250.10124080733469401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)