FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29128
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT605220.777258994781654No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT22067.573468827245263TruSeq Adapter, Index 3 (95% over 21bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG16725.740181268882175TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT15715.393435869266685RNA PCR Primer, Index 3 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGT6542.245262290579511No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT6092.090771765998352TruSeq Adapter, Index 3 (96% over 26bp)
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA5872.0152430650920077RNA PCR Primer, Index 3 (96% over 28bp)
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG3731.2805547926393848TruSeq Adapter, Index 3 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGT2410.8273825872013183No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTT2360.8102169733589673No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC1970.6763251853886295Illumina PCR Primer Index 12 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC1830.6282614666300467No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT1820.6248283438615765No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC1790.6145289755561659No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCT1530.5252677835759407Illumina PCR Primer Index 8 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC1120.38450975006866245No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.36391101345784127No Hit
TCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCTTG1050.3604778906893711Illumina PCR Primer Index 8 (96% over 26bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAGG1000.34331227684702004No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAACCTCGTATGCCGT900.30898104916231806No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTT870.29868168085690744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.2883823125514968No Hit
CTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC710.24375171656138422No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAG640.21971985718209283No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTTCT590.2025542433397418Illumina PCR Primer Index 8 (95% over 21bp)
CTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCTTGA570.19568799780280144Illumina PCR Primer Index 8 (96% over 26bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACGTAGAGG530.18195550672892064No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATATCGTATGCCGT510.17508926119198023No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCT510.17508926119198023Illumina PCR Primer Index 8 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.16478989288656962No Hit
ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT480.16478989288656962TruSeq Adapter, Index 3 (96% over 27bp)
TCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTTCTGCTTG440.1510574018126888Illumina PCR Primer Index 8 (96% over 26bp)
TCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAA370.12702554243339742TruSeq Adapter, Index 3 (96% over 28bp)
CACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTG350.12015929689645703Illumina PCR Primer Index 8 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAACCTCGTATGCCGTCTT300.10299368305410601No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)