FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98488
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT52015.280846397530663No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT19261.9555681910486555RNA PCR Primer, Index 46 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT13211.3412801559580863RNA PCR Primer, Index 46 (95% over 23bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG10901.1067338152871415RNA PCR Primer, Index 46 (96% over 28bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT5280.5361059215335878RNA PCR Primer, Index 46 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGT5220.5300138087888879No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3950.4010640890260742No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA3700.3756802859231581RNA PCR Primer, Index 46 (96% over 28bp)
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG3220.3269433839655592TruSeq Adapter, Index 4 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.31374380635204285No Hit
GTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGCAATCTCGGCTCAC3050.30968239785557633No Hit
ATATTGGGCATAATCGTATCATTCAAGATTAAGTCTGGGTTATTTCTTTG2100.21322394606449516No Hit
AATTAAGGAACAGCAATTTAGAAAGAAACACACTTTAAGAAATCAAAATT1860.1888554950856957No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAGCTCGTATGCCGT1860.1888554950856957No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGTCTT1830.1858094387133458No Hit
ACTTAATATTGGGCATAATCGTATCATTCAAGATTAAGTCTGGGTTATTT1740.176671269596296No Hit
GGCTTAGGCAGAAGCCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCT1680.17057915685159614No Hit
ATCTTAAATAGATCTTAGGCAAAATACCAGCTGATGAAGGCATCTGATGC1670.1695638047274795No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC1660.16854845260336285TruSeq Adapter, Index 18 (95% over 24bp)
GTACATGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACA1650.1675331004792462No Hit
CTTCCAGGACATCAAGAAGCCAGCTGAAGATGAGTGGGGTAAAACCCCAG1650.1675331004792462No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC1650.1675331004792462No Hit
GTACATGGGCTGGACAAGTGCCACTACAACAACACTAAACCTGAACTTTT1630.16550239623101293No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC1580.1604256356104297No Hit
GGGCATAATCGTATCATTCAAGATTAAGTCTGGGTTATTTCTTTGATAAG1540.1563642271139631No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT1540.1563642271139631No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.15027211436926327No Hit
CCTCCATACCGGGCTGATTTTTGTATTTTTAGTGGAGATGGGGTTTCGCC1480.15027211436926327No Hit
GAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCCTCCGATTTCCT1460.14824141012102998No Hit
GTCTAAGATTGGTCATACATTCCCAATTTACTCTCAGTTCCAGTCAAGCT1440.1462107058727967No Hit
CTCCGGGACCATCTTCTCGGCCATCTCCTGCTTCTGGGACCTGCCAGCAC1420.1441800016245634No Hit
GAACTGAATGTGGCCGGGCGCAGTGGCTCACGCCTGTAATCCCAGCACTT1360.13808788887986354No Hit
GGCTACGAGCGTCTCCTGAAGATGCAAAACCAGCGTGGCGGCCGCGCTCT1340.13605718463163025No Hit
TCCTGGAAGAGAGCGCGGCCGCCACGCTGGTTTTGCATCTTCAGGAGACG1330.13504183250751362No Hit
GTCTTGTTCTGTTGCCCAGGCTGGAGTGCAGTGGTGCAGTCTTGGCTCAC1300.13199577613516367No Hit
TCTAGGAAGTGAGTCTCCAGGAAGTCACAGAGATGGGGGTCCGTGCGGGC1270.12894971976281375No Hit
GGCTGGAGTGCAGTGGCGCAATCTCGGCTCACTGCAACCTCCACCTCCCG1240.1259036633904638No Hit
TTCCAGAGCCACATCATCGCGGTCGAAATAGAAGCCCAGAGAGAGGTAGG1230.12488831126634718No Hit
GTACATGGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCT1230.12488831126634718No Hit
CCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCT1190.1208269027698806No Hit
CTCGTAGCCCTCGCGCTTCTCCTCGGCCAGTTCGCGGAAGAAGTGGCTCA1180.11981155064576396No Hit
GTGTTAGCCAGGATGGTCTCGATCTCCTGACCTTGTGATCCACCCACCTC1180.11981155064576396No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA1170.1187961985216473No Hit
CTCTTCCAGGACATCAAGAAGCCAGCTGAAGATGAGTGGGGTAAAACCCC1130.11473479002518074No Hit
AAATAGATCTTAGGCAAAATACCAGCTGATGAAGGCATCTGATGCCTTCA1130.11473479002518074No Hit
GAGTACATGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAA1130.11473479002518074No Hit
CTGCTGGATGTACCTTTTTTCTGAGCTCTGGTTTGCCTTTCTTGACTGTG1120.11371943790106408No Hit
GGGTTTCACCGTGTTAGCCAGGATGGTCTCGATCTCCTGACCTTGTGATC1110.11270408577694746No Hit
GTGTTATATTGCCTGGGCAGGTCTCGAAATCCTGGGCTCAAGCTATCCTC1090.11067338152871416No Hit
CACTTTAAGAAATCAAAATTCTCAATTCAGGCAGTCTGTTTCTATCATTT1090.11067338152871416No Hit
CTTGTAAGCTCATCTGCCTCAGATTTAATCATAATACATGAATTAAATTA1080.10965802940459751No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC1080.10965802940459751No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGTCTTCT1070.10864267728048088Illumina PCR Primer Index 8 (95% over 21bp)
ATCCAGCAGTGGTCATTCGACAACGAAAGTCATACCGTAGAAAAGATGGC1060.10762732515636422No Hit
GATTAAGTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCA1060.10762732515636422No Hit
TCCTAGATGAGGAAGTGAAGCTTATCAAGAAGATGGGTGACCACCTGACC1060.10762732515636422No Hit
ATTCAAGATTAAGTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCG1050.10661197303224758No Hit
GAATTAAATTAATCAAATTAAGGAACAGCAATTTAGAAAGAAACACACTT1040.10559662090813093No Hit
GAATGTGGCCGGGCGCAGTGGCTCACGCCTGTAATCCCAGCACTTTGGGA1020.10356591665989766No Hit
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC1020.10356591665989766No Hit
GTCTTGGCTCACTGCAACCTCTGCCTCCTGGGTTCAATCCGTTCTCCTGC1000.10153521241166436No Hit
GTATTCTACTCCTTTAAAAATTTCATATTGCCCAACAAAAAGTGGTTATT990.10051986028754772No Hit
GTTCAGTCATCTCCAAAAACAGTAAAAATAACCACTTTTTGTTGGGCAAT990.10051986028754772No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)