FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98488
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGCAATCTCGGCTCAC2930.2974981723661766No Hit
ATATTGGGCATAATCGTATCATTCAAGATTAAGTCTGGGTTATTTCTTTG2480.2518073267809276No Hit
ACTTAATATTGGGCATAATCGTATCATTCAAGATTAAGTCTGGGTTATTT1970.2000243684509788No Hit
ATCTTAAATAGATCTTAGGCAAAATACCAGCTGATGAAGGCATCTGATGC1860.1888554950856957No Hit
CTTCCAGGACATCAAGAAGCCAGCTGAAGATGAGTGGGGTAAAACCCCAG1770.17971732596864592No Hit
GGCTACGAGCGTCTCCTGAAGATGCAAAACCAGCGTGGCGGCCGCGCTCT1730.17565591747217935No Hit
AATTAAGGAACAGCAATTTAGAAAGAAACACACTTTAAGAAATCAAAATT1700.17260986109982943No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC1560.15839493136219643No Hit
TCCTGGAAGAGAGCGCGGCCGCCACGCTGGTTTTGCATCTTCAGGAGACG1490.1512874664933799No Hit
GGCTTAGGCAGAAGCCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCT1480.15027211436926327No Hit
GGGCATAATCGTATCATTCAAGATTAAGTCTGGGTTATTTCTTTGATAAG1480.15027211436926327No Hit
GAACTGAATGTGGCCGGGCGCAGTGGCTCACGCCTGTAATCCCAGCACTT1390.14113394525221348No Hit
GTACATGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACA1380.14011859312809682No Hit
GGCTAACACGGTGAAACCCTGTCTCTACTAAAAATACAAAAAATTAGCCG1370.13910324100398017No Hit
GGGTTTCACCGTGTTAGCCAGGATGGTCTCGATCTCCTGACCTTGTGATC1320.13402648038339696No Hit
CTCCTACACCTACCTCTCTCTGGGCTTCTATTTCGACCGCGATGATGTGG1320.13402648038339696No Hit
TTCCAGAGCCACATCATCGCGGTCGAAATAGAAGCCCAGAGAGAGGTAGG1300.13199577613516367No Hit
CCTCCATACCGGGCTGATTTTTGTATTTTTAGTGGAGATGGGGTTTCGCC1270.12894971976281375No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA1270.12894971976281375No Hit
GTGAAACCCTGTCTCTACTAAAAATACAAAAAATTAGCCGGGCGTGGTGG1270.12894971976281375No Hit
TCTAGGAAGTGAGTCTCCAGGAAGTCACAGAGATGGGGGTCCGTGCGGGC1260.1279343676386971No Hit
GGCTGGAGTGCAGTGGCGCAATCTCGGCTCACTGCAACCTCCACCTCCCG1250.12691901551458046No Hit
GTCTAAGATTGGTCATACATTCCCAATTTACTCTCAGTTCCAGTCAAGCT1250.12691901551458046No Hit
GTGTTATATTGCCTGGGCAGGTCTCGAAATCCTGGGCTCAAGCTATCCTC1240.1259036633904638No Hit
AAATAGATCTTAGGCAAAATACCAGCTGATGAAGGCATCTGATGCCTTCA1230.12488831126634718No Hit
GATAAGAACTGAATGTGGCCGGGCGCAGTGGCTCACGCCTGTAATCCCAG1200.12184225489399723No Hit
ATCCTGGCTAACACGGTGAAACCCTGTCTCTACTAAAAATACAAAAAATT1160.11778084639753066No Hit
CCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCT1150.11676549427341402No Hit
GAGTACATGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAA1140.11575014214929738No Hit
GTGTATAAGCATATCAATATTAAAAAGCAAGCAAGCAGAATTTGGAATTC1130.11473479002518074No Hit
GTCTTGTTCTGTTGCCCAGGCTGGAGTGCAGTGGTGCAGTCTTGGCTCAC1130.11473479002518074No Hit
GGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCTCACG1130.11473479002518074No Hit
GTACATGGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCT1130.11473479002518074No Hit
GTGTTAGCCAGGATGGTCTCGATCTCCTGACCTTGTGATCCACCCACCTC1120.11371943790106408No Hit
CACTTTAAGAAATCAAAATTCTCAATTCAGGCAGTCTGTTTCTATCATTT1110.11270408577694746No Hit
TCCTAGATGAGGAAGTGAAGCTTATCAAGAAGATGGGTGACCACCTGACC1100.1116887336528308No Hit
GATTAAGTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCA1090.11067338152871416No Hit
ATTCTAAGCAGAGTATGTAAATTGGAAGTTAACTTATGCACGCTTAACTA1070.10864267728048088No Hit
GAATTAAATTAATCAAATTAAGGAACAGCAATTTAGAAAGAAACACACTT1060.10762732515636422No Hit
GTTTTACCCCACTCATCTTCAGCTGGCTTCTTGATGTCCTGGAAGAGAGC1050.10661197303224758No Hit
CTCGTAGCCCTCGCGCTTCTCCTCGGCCAGTTCGCGGAAGAAGTGGCTCA1050.10661197303224758No Hit
GATTACGGGCGCCCGCCACCACGCCCGGCTAATTTTTTGTATTTTTAGTA1040.10559662090813093No Hit
GATGGGGGTCCGTGCGGGCAGAACCCAGGGCATGAAGATCCAAAAGGGCC1030.1045812687840143No Hit
GAATGTGGCCGGGCGCAGTGGCTCACGCCTGTAATCCCAGCACTTTGGGA1030.1045812687840143No Hit
CTGCTGGATGTACCTTTTTTCTGAGCTCTGGTTTGCCTTTCTTGACTGTG1020.10356591665989766No Hit
ATTCAAGATTAAGTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCG990.10051986028754772No Hit
GTACATGGGCTGGACAAGTGCCACTACAACAACACTAAACCTGAACTTTT990.10051986028754772No Hit
GTTTAATTGTGACATTTTAAAATTGTACAGGTATTTTGACATCTGCCCCC990.10051986028754772No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)