FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001420811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396507
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT90752.288736390530306No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT30150.7603901066059363TruSeq Adapter, Index 11 (95% over 21bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG27490.6933042796217974TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT20420.5149972131639542RNA PCR Primer, Index 11 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19540.4928034057406301No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17440.4398409107531519No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA10850.27363955743530377RNA PCR Primer, Index 11 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT7980.201257480952417TruSeq Adapter, Index 11 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGT7560.19066498195492135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7540.19016057724075489No Hit
GTACATGGGAGTCCCTATTTCCTTTGCTGTGTTCCCCAATGAAGATGGCT5960.15031260482160466No Hit
GTACATGGGAATGAAGAAAGTGAGTTGGATATTTTTAAAGCAGTTAACTA5390.1359370704678606No Hit
GTATATGGAACATGCTTTTTTCAGAAAATTGGCAGTAACTGACTTTGAAG5190.130893023326196No Hit
GTACATGGAGGTGATGGTGTTTCTTAGAGAGAAGATCACTGAAGAAACTT4390.11071683475953767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4320.10895141825995505No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG4270.1076904064745389TruSeq Adapter, Index 11 (95% over 24bp)
GTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTG4210.10617719233203954No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA4190.10567278761787309No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT4040.10188975226162464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4030.10163754990454141No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)