FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73072
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT62228.514889424129626No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT20052.743869060652507RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT13361.8283336982701994RNA PCR Primer, Index 27 (96% over 25bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG11841.6203196846945478RNA PCR Primer, Index 27 (96% over 30bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT5800.7937376833807751RNA PCR Primer, Index 27 (96% over 28bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA4190.5734070505802497RNA PCR Primer, Index 27 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGT3950.5405627326472521No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.5077184147142544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.41602802715130277No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG3000.4105539741624699RNA PCR Primer, Index 27 (100% over 24bp)
GCCTTGGTACAGTGCGCCTGGGTTTGTCAAGGCTGCTGCTACAAAGCTGG2900.39686884169038755No Hit
GTACATGGGAAACCTCATTTTTTTTGTTCCTTTTCTTGTTACTTTTAAGA2630.3599189840157653No Hit
GTACATGGGAGGCCACCTTCCTTCTGGCAGGGACTCTTATTTATTCCCAT2460.3366542588132253No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC2250.3079154806218524No Hit
GTACATGGGAAGGGCACAAGTCCTGGATGTTGTGTGTGTCGAGAGGCCAA2090.2860192686665207No Hit
GTACATGGGCAATGGTCAGTCTTTTCCTGGCCAGAGGAAAGATTGATGGC2080.28465075541931245No Hit
GCTCTAGGGCTTTCGGTTTCCCCTTCTTCCGGTAGGCCGCGTAGAGGCAT2060.281913728924896No Hit
GTACATGGGACAGCCCGAGTGCAAAAAGCGCCTCAATGACCGGATTGAGA1920.26275454346398075No Hit
GGGTAGGAGCACATGATGCATCCTTCAGCCACTGCAACCTCCAGAGGGAC1720.23538427851981608No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC1680.22991022553098314No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGT1670.22854171228377493No Hit
GTACATGGGGAAGCCCGGAACTGCTGGGCCACCCGTGGTCTCTGTGTCCT1670.22854171228377493No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGC1670.22854171228377493TruSeq Adapter, Index 27 (96% over 25bp)
CCCTGGTACCGTGTAGTTGCCGAAGTCCAGATCTGCCATGGCAAAACGGA1610.22033063280052553No Hit
GGTATCACCATCTGACATATTTTCCCTTTTCCGGCGTTGTAGTAGCACTG1590.21759360630610905No Hit
CATTTGGCCTCTGTGACCCAGAAAAGCGGTAGAGCAGGGTAGGAGCACAT1570.21485657981169257No Hit
GTACATGGGACACATGATGATGGATACATGATCTGTTGTGACAAATGCAG1570.21485657981169257No Hit
ATGTTGGAGTATGCTGAAATGCAGTATATAATTCCACAGGAACCTCATCA1530.20938252682285965No Hit
CTTTTAATGTGATAACTTTCCACACCCTCTCCACGTAGAGGCACAAAGGA1500.20527698708123496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.2039084738340267No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT1440.19706590759798556RNA PCR Primer, Index 27 (95% over 22bp)
ATATAATTCCACAGGAACCTCATCACCACTCTCAGTTGCACTGGTATCAC1430.19569739435077732No Hit
AGCTAAAAGAGCAAGAGTACACATCAACAAAAATGGAAACAAGGCTTTGG1400.19159185460915262No Hit
TCTGTATATTGTTCCTGATTGGCCTGATGTGTGGACGGATCACCAAGCGA1350.18474928837311147No Hit
TATTTATTCAATGCTCTCACATTTGGCCTCTGTGACCCAGAAAAGCGGTA1350.18474928837311147No Hit
CTCTTGAAGCAGTTAAAGTAATTGATGTTGGAGTATGCTGAAATGCAGTA1350.18474928837311147No Hit
CTCTTATTTATTCCCATTGCTCTAGGGCTTTCGGTTTCCCCTTCTTCCGG1330.182012261878695No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTT1320.18064374863148674No Hit
ACATGGGACAGCCCGAGTGCAAAAAGCGCCTCAATGACCGGATTGAGATG1310.17927523538427853No Hit
TCATTGGTCTGGTCCCCGTCAAAGTTTCCACAGGCCCCACACAGTTTCCC1300.1779067221370703No Hit
GCCATAACATGGGGAGAAGTCCTGCGCTCTCCATTTCTCCATCGCTGGCT1280.17516969564265383No Hit
GTTGTGACAAATGCAGCGTTTGGCAACATATTGACTGCATGGGGATTGAT1270.1738011823954456No Hit
GTACATGGGGGTTGGAGAAAGGAGAGAAAGGAAAGCGCGAGGAGCCGCCG1240.1696956426538209No Hit
GTTTATTAATATTTGGTGACAAAAGAACTTAAATTGACCGAAAATCAAAA1240.1696956426538209No Hit
GTCCTGGATGTTGTGTGTGTCGAGAGGCCAAAGGCTGGTGGCAAGTGCAC1240.1696956426538209No Hit
GTATATAATTCCACAGGAACCTCATCACCACTCTCAGTTGCACTGGTATC1210.1655901029121962No Hit
CATATATAGTATGTCTCCATCAAGTGAAACTTCATCACCTTTTTACAATT1210.1655901029121962No Hit
GTGTTAGCCAGAATGGTCTCAATCTCCAGACCTCGTGATCCGCCCGCCCT1200.16422158966498795No Hit
GTTTTATTTCTAGACTTTCATGTTTGTCTTTTTGTCTTCTGCTGGAAACA1200.16422158966498795No Hit
ATACATATCTATGTGAACGTTGTCAGCCTAGGAATTTGGATAAAGAGAGG1180.16148456317057147No Hit
GCTTAAGATATTTATTCAATGCTCTCACATTTGGCCTCTGTGACCCAGAA1140.15601051018173856No Hit
CATATTGACTGCATGGGGATTGATAGGCAGCATATTCCTGATACATATCT1140.15601051018173856No Hit
TATTTATTCCCATTGCTCTAGGGCTTTCGGTTTCCCCTTCTTCCGGTAGG1140.15601051018173856No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1120.15327348368732208No Hit
GTATCAACGCAGAGTACATGGGCAATGGTCAGTCTTTTCCTGGCCAGAGG1100.15053645719290562No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG1100.15053645719290562No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTACCTCGTATGCCGT1080.14779943069848916No Hit
TGTTATGGCTGATCAGTCATCCACCAGGAACGAAGATTTCCTGAAGAAGA1080.14779943069848916No Hit
TGTCTGGAGTGTGCAGAAAGGTCAGGCTGGACTGCCTGTCACATCCTGAG1060.1450624042040727No Hit
ACAGAATACCATCCCCTGGTACCGTGTAGTTGCCGAAGTCCAGATCTGCC1050.14369389095686447No Hit
GTATTGTAGCCTGTCCTTGAGCTCTCGTGTCACTCGCTTGGTGATCCGTC1040.14232537770965623No Hit
CCTTGGTACAGTGCGCCTGGGTTTGTCAAGGCTGCTGCTACAAAGCTGGA1010.13821983796803153No Hit
CCTGATGGCTCCCTGCTAGTCCGCCAGAAGGCAGGGGTCCAGGTGTGGCT980.13411429822640683No Hit
GTACATGGGATTGCAATCTCACTGTGGTATTTTATAGAAGCTATTACAAC970.1327457849791986No Hit
GTATCAACGCAGAGTACATGGGACACATGATGATGGATACATGATCTGTT960.13137727173199037No Hit
CACATACACTCTAGCTAAAAGAGCAAGAGTACACATCAACAAAAATGGAA950.13000875848478213No Hit
CTTTACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC940.1286402452375739RNA PCR Primer, Index 27 (95% over 21bp)
GTGGGGGGTTTGACTTTATTTGAAATTTTTGGTAGATTGTTCATTTGGTT930.12727173199036568No Hit
GTCCTGCGCTCTCCATTTCTCCATCGCTGGCTTCTCCTGGGAGTCATGCC930.12727173199036568No Hit
GTCCTGGAGCTGTGAGGAGATTCGGGCCGTCACCCTGCCTCCCCTGCGTC920.12590321874315744No Hit
CCCTTGTTTGGAGTCAACGTCACCATCCCATCCTGGAAGAAGATGTGGAC920.12590321874315744No Hit
CTCCAGAGGGACCAGGTCTTCTTCAGGAAATCTTCGTTCCTGGTGGATGA910.1245347054959492No Hit
ATATACAGATGCTCCCACTGATCACAGAGAGAATGTAGTTCTTAGATGGA910.1245347054959492No Hit
AAACAAGGGTGTGTGGGTGAATGGTCTCCGAGTGGATCTCCCAGCTGAGA910.1245347054959492No Hit
GTACCAGGGGATGGTATTCTGTAGTCCTGGGCAGCGGGAAGAGAGCTCAT890.12179767900153274No Hit
GTAGTAGCACTGCCCTCTCTTTATCCAAATTCCTAGGCTGACAACGTTCA890.12179767900153274No Hit
GAGTACATGGGACACATGATGATGGATACATGATCTGTTGTGACAAATGC880.1204291657543245No Hit
GAGTACATGGGAGGCCACCTTCCTTCTGGCAGGGACTCTTATTTATTCCC880.1204291657543245No Hit
ATACAATGATGTATTTCTTTATTTTCACATACACTCTAGCTAAAAGAGCA870.11906065250711627No Hit
ATTCAATGCTCTCACATTTGGCCTCTGTGACCCAGAAAAGCGGTAGAGCA870.11906065250711627No Hit
GTATCAACGCAGAGTACATGGGAAGGGCACAAGTCCTGGATGTTGTGTGT870.11906065250711627No Hit
GCATATTCCTGATACATATCTATGTGAACGTTGTCAGCCTAGGAATTTGG850.11632362601269981No Hit
GTATCAACGCAGAGTACATGGGAAACCTCATTTTTTTTGTTCCTTTTCTT850.11632362601269981No Hit
GGTATCAACGCAGAGTACATGGGAAGGGCACAAGTCCTGGATGTTGTGTG840.11495511276549157No Hit
GTGTGGGGCCTGTGGAAACTTTGACGGGGACCAGACCAATGATTGGCATG830.11358659951828333No Hit
AACTTAAATTGACCGAAAATCAAAAGTTACATTGCCTTGGTACAGTGCGC820.11221808627107512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG820.11221808627107512No Hit
GTCTTTTCCTGGCCAGAGGAAAGATTGATGGCCCTCCCACTTGAACTGAC820.11221808627107512No Hit
CATGATGATGGATACATGATCTGTTGTGACAAATGCAGCGTTTGGCAACA810.11084957302386687No Hit
GTATATTGTTCCTGATTGGCCTGATGTGTGGACGGATCACCAAGCGAGTG800.10948105977665863No Hit
TTTCTAGACTTTCATGTTTGTCTTTTTGTCTTCTGCTGGAAACATGCCGG780.10674403328224218No Hit
GTATCAACGCAGAGTACATGGGAGGCCACCTTCCTTCTGGCAGGGACTCT780.10674403328224218No Hit
GTATCAACGCAGAGTACATGGGACAGCCCGAGTGCAAAAAGCGCCTCAAT780.10674403328224218No Hit
GGGCGAAGCAGAGGCAAAGCGGCCCAGCCCAACAGCCGGGATGGGGCAGG770.10537552003503393No Hit
GGTACAGTGCGCCTGGGTTTGTCAAGGCTGCTGCTACAAAGCTGGAGAAC750.10263849354061748No Hit
GAGTACATGGGCAATGGTCAGTCTTTTCCTGGCCAGAGGAAAGATTGATG750.10263849354061748No Hit
GTTTCCCAGCATGGTCATTGCTGACAATCACAGCCACCTTCCCATTGGCT750.10263849354061748No Hit
GTATCAACGCAGAGTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.10263849354061748No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)