FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001420825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132437
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT70525.324795940711432No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT23041.7396951003118464RNA PCR Primer, Index 31 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT16011.2088766734371814RNA PCR Primer, Index 31 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14541.0978805016724933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11600.875888158143117No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG10200.7701775183672236RNA PCR Primer, Index 31 (96% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6100.46059635902353574No Hit
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT5710.43114839508596536RNA PCR Primer, Index 31 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGT4620.34884511126044837No Hit
GTCTAGAGCTTGTCTCAATGGATCTAGAACTTCATCGCCCTCTGATCGCC4270.32241745131647503No Hit
GTCTTGCTCTGTTGCCCAGGCTTCAATGCAGTGGTACGATCGCGATCACA3920.29598979137250164No Hit
ATTATATGGTTGTTACACTCGGCCACAAATAAACACAGAAATAGTCCAGA3560.26880705543012906No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG3480.26276644744293515RNA PCR Primer, Index 38 (95% over 24bp)
GTCTTGGTTCCCCGGAGACGTCCAGTCCGGCGGGCAGCAATGAGACCCAC2980.22501264752297317No Hit
GTACATGGGACGGGGTGTGGCCATGAATCCTGTGGAGCATCCTTTTGGAG2950.22274741952777546No Hit
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA2780.2099111275549884RNA PCR Primer, Index 31 (96% over 29bp)
GGTTTGTAGAGGCTTACTTAACCTTAATGGAAACGCTGGAAATCTGCAGG2610.19707483558220135No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG2460.18574869560621277No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCC2370.17895301162061963No Hit
GTACATGGGGACCTTAAAGCAGCAGGATGGTCGGAATTAAGTGAGAGAGA2370.17895301162061963No Hit
GTACATGGGAGGCTGAGGTGGGAAGATCACTGGAGCCCAGGAGTTCTGGG2290.1729124036334257No Hit
ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC2230.16838194764303027RNA PCR Primer, Index 38 (96% over 25bp)
ATTTAATACATCAAAATGTGGGATTTCTTGACCTTGCTTCTCTCCTGGTC2230.16838194764303027No Hit
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT2200.16611671964783256RNA PCR Primer, Index 31 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC2040.15403550367344473No Hit
TACTTAACCTTAATGGAAACGCTGGAAATCTGCAGGGGGAGGGAGAGGGG2030.1532804276750455No Hit
GTACATGGGATCTTATTGGAGGCTAATGCAGCTGGTGACTTTAAGCTGAA2020.15252535167664627No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1950.14723981968785158No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTAGCTCGTATGCCGT1930.14572966769105308No Hit
GTCCAGGGCAGAGGACCAACATGGGCATTTTGTTTATGAGCAAGGTGGGT1880.1419542876990569No Hit
GTGCAGGGTGAGGACAGAAAAATGCAAAGGAGTCTGAGGCCTAGAGACAC1870.14119921170065766No Hit
CCAATAATGTGGGTTTATGATTTTCACTATGTGCAGGGTGAGGACAGAAA1850.1396890597038592No Hit
CTATAAACCACACCAATAATGTGGGTTTATGATTTTCACTATGTGCAGGG1780.13440352771506453No Hit
ATTTAATACATCAAAATGTGGGATTTCTTGACCTTGCTTCTCTCCTGGCC1780.13440352771506453No Hit
CTCCTGCACAGTCTTGGTTCCCCGGAGACGTCCAGTCCGGCGGGCAGCAA1770.13364845171666528No Hit
GTTGTTACACTCGGCCACAAATAAACACAGAAATAGTCCAGAATGTCACA1630.12307738773907594No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.12081215974387823Clontech SMART CDS Primer II A (100% over 21bp)
GTACATGGGCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA1590.12005708374547898No Hit
CCTTAAAGCAGCAGGATGGTCGGAATTAAGTGAGAGAGATGCCATCTACA1580.11930200774707975No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.11779185575028125No Hit
GTCTTGCTGTGTTGCCCAGTTTGGCCTTGAGCTCTTGGGCTCATACAATC1550.11703677975188204No Hit
GTACATGGGAGCCGGGTGTGATGGCACGCACCTGTAGTCCCAGCTACTTG1550.11703677975188204No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1550.11703677975188204No Hit
GTACATGGGATCTGAAGTAAAAAATTCACTGAATGAGCTTAACAGAAAAT1540.11628170375348279No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1530.11552662775508354No Hit
ACGCAGAGGTTGCAGTGAGTGGAGATTGCACCACTGCACTCCAGCCAGGG1500.11326139975988583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1490.11250632376148659No Hit
GAGTACATGGGGACCTTAAAGCAGCAGGATGGTCGGAATTAAGTGAGAGA1470.11099617176468812No Hit
GCATGCTACTGTCTAGAGCTTGTCTCAATGGATCTAGAACTTCATCGCCC1460.11024109576628888No Hit
GTTGTATACATTGAACCATTCTGGGTGATGATTCATCTTCTCTGCTTGTA1460.11024109576628888No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT1410.10646571577429269No Hit
GAGTACATGGGACGGGGTGTGGCCATGAATCCTGTGGAGCATCCTTTTGG1410.10646571577429269No Hit
CCTCTGAGACCCACCTTGCTCATAAACAAAATGCCCATGTTGGTCCTCTG1410.10646571577429269No Hit
GTCCAGAATGTCACAGGTCCAGGGCAGAGGACCAACATGGGCATTTTGTT1410.10646571577429269No Hit
GCTAAAACAGCGGAAGAGGTGATTTATTATATGGTTGTTACACTCGGCCA1400.10571063977589344No Hit
GGCTTCAATGCAGTGGTACGATCGCGATCACAGTTCACTTCAACCTCAAC1380.10420048777909498No Hit
TCCAAAAGCTATGTAATAAACCAAAATTTAATGTTCCTGCTTTATAAGTA1380.10420048777909498No Hit
ACTTTATTGAGGCCCTCAGCACTAGTTCTCTTTCTCCTGCACAGTCTTGG1370.10344541178069573No Hit
GTACAAGATTTTACATGTATTTTGGAAGAAATCACACAGAAGCAGCTGCT1360.10269033578229649No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT1330.10042510778709877No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)