FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001420826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132437
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGAGCTTGTCTCAATGGATCTAGAACTTCATCGCCCTCTGATCGCC4890.36923216321722785No Hit
ATTATATGGTTGTTACACTCGGCCACAAATAAACACAGAAATAGTCCAGA3920.29598979137250164No Hit
GTACATGGGACGGGGTGTGGCCATGAATCCTGTGGAGCATCCTTTTGGAG3310.24993015547014807No Hit
GTCTTGCTCTGTTGCCCAGGCTTCAATGCAGTGGTACGATCGCGATCACA2970.22425757152457396No Hit
GTACATGGGAGGCTGAGGTGGGAAGATCACTGGAGCCCAGGAGTTCTGGG2700.20387051956779448No Hit
GTCTTGGTTCCCCGGAGACGTCCAGTCCGGCGGGCAGCAATGAGACCCAC2590.19556468358540288No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2570.1940545315886044No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG2390.1804631636174181No Hit
GTACATGGGGACCTTAAAGCAGCAGGATGGTCGGAATTAAGTGAGAGAGA2320.17517763162862343No Hit
GGTTTGTAGAGGCTTACTTAACCTTAATGGAAACGCTGGAAATCTGCAGG2310.17442255563022419No Hit
GTGCAGGGTGAGGACAGAAAAATGCAAAGGAGTCTGAGGCCTAGAGACAC2040.15403550367344473No Hit
GTCCAGGGCAGAGGACCAACATGGGCATTTTGTTTATGAGCAAGGTGGGT2030.1532804276750455No Hit
GTACATGGGATCTTATTGGAGGCTAATGCAGCTGGTGACTTTAAGCTGAA1880.1419542876990569No Hit
ACGCAGAGGTTGCAGTGAGTGGAGATTGCACCACTGCACTCCAGCCAGGG1860.14044413570225842No Hit
CTATAAACCACACCAATAATGTGGGTTTATGATTTTCACTATGTGCAGGG1830.1381789077070607No Hit
ATTTAATACATCAAAATGTGGGATTTCTTGACCTTGCTTCTCTCCTGGTC1820.1374238317086615No Hit
CCAATAATGTGGGTTTATGATTTTCACTATGTGCAGGGTGAGGACAGAAA1770.13364845171666528No Hit
TACTTAACCTTAATGGAAACGCTGGAAATCTGCAGGGGGAGGGAGAGGGG1760.13289337571826604No Hit
GTACATGGGCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA1710.12911799572626986No Hit
GGTATCAACGCAGAGGTTGCAGTGAGTGGAGATTGCACCACTGCACTCCA1630.12307738773907594No Hit
GTTGTATACATTGAACCATTCTGGGTGATGATTCATCTTCTCTGCTTGTA1620.1223223117406767No Hit
GTACATGGGAGCCGGGTGTGATGGCACGCACCTGTAGTCCCAGCTACTTG1610.12156723574227746No Hit
GCATGCTACTGTCTAGAGCTTGTCTCAATGGATCTAGAACTTCATCGCCC1610.12156723574227746No Hit
GTTCTGGGCTGTGCGCTATGCTGATTAGGTGTCCACACTAAGTTCTGCAT1610.12156723574227746No Hit
ATTTAATACATCAAAATGTGGGATTTCTTGACCTTGCTTCTCTCCTGGCC1540.11628170375348279No Hit
CACCAATAATGTGGGTTTATGATTTTCACTATGTGCAGGGTGAGGACAGA1530.11552662775508354No Hit
GAAATAAGTCATTTATTTAATACATCAAAATGTGGGATTTCTTGACCTTG1490.11250632376148659No Hit
GTACATGGGATCTGAAGTAAAAAATTCACTGAATGAGCTTAACAGAAAAT1480.11175124776308737No Hit
CTCCTGCACAGTCTTGGTTCCCCGGAGACGTCCAGTCCGGCGGGCAGCAA1460.11024109576628888No Hit
GTCTTGCTGTGTTGCCCAGTTTGGCCTTGAGCTCTTGGGCTCATACAATC1440.1087309437694904No Hit
GTTGTTACACTCGGCCACAAATAAACACAGAAATAGTCCAGAATGTCACA1430.10797586777109115No Hit
CCTCTGAGACCCACCTTGCTCATAAACAAAATGCCCATGTTGGTCCTCTG1420.10722079177269192No Hit
CCTTAAAGCAGCAGGATGGTCGGAATTAAGTGAGAGAGATGCCATCTACA1410.10646571577429269No Hit
ATCTAGAACTTCATCGCCCTCTGATCGCCGATCACCTCTGAGACCCACCT1410.10646571577429269No Hit
GCTAAAACAGCGGAAGAGGTGATTTATTATATGGTTGTTACACTCGGCCA1400.10571063977589344No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT1390.1049555637774942No Hit
ACTTTATTGAGGCCCTCAGCACTAGTTCTCTTTCTCCTGCACAGTCTTGG1370.10344541178069573No Hit
CTACTGTCTAGAGCTTGTCTCAATGGATCTAGAACTTCATCGCCCTCTGA1350.10193525978389724No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1340.10118018378549802No Hit
GAGTACATGGGACGGGGTGTGGCCATGAATCCTGTGGAGCATCCTTTTGG1330.10042510778709877No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)