FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001420833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511193
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT197203.85764280809792No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120722.361534684551627No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT93881.8364883713196385TruSeq Adapter, Index 11 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90561.771542255077828No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71871.4059269199695614No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT59081.1557278757729468RNA PCR Primer, Index 11 (95% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24450.47829293437116704Clontech SMART CDS Primer II A (100% over 21bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG21980.4299745888539162TruSeq Adapter, Index 11 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT18370.3593554684825496TruSeq Adapter, Index 11 (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16900.3305992061706635No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG15290.29910425221002634TruSeq Adapter, Index 11 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC11450.2239858527014259No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT9530.18642665294712563No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9060.17723247384060423No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8780.17175509054310212No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7290.1426075865671087No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC6480.1267622991707633RNA PCR Primer, Index 17 (95% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6190.12108929504120752Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA6000.11737249923218823RNA PCR Primer, Index 11 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5830.11404694508727624No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5660.11072139094236425Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5650.11052577011031058No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA5550.10856956178977413No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)