FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001420834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511193
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58141.137339517559904No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13740.26878302324171105No Hit
CATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTCGCCGT7380.14436817405559152Illumina RNA PCR Primer (95% over 24bp)
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG6990.13673896160549928No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6900.13497837411701646Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6200.12128491587326116Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)