FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129082
Sequences flagged as poor quality0
Sequence length100
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT2539319.671991447297067No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT125459.718628468725306TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT68285.289660835747819RNA PCR Primer, Index 1 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65395.0657721448381645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60174.661378038766055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40553.1414139849088176No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26842.0792984304550597No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22411.736105731240607No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20501.5881377728885513No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC16301.2627632047845556No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG15391.1922653816953563TruSeq Adapter, Index 1 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT13811.0698625679800435No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9600.7437132985234192No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9300.7204722579445624Clontech SMART CDS Primer II A (100% over 21bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG8950.6933577106025627TruSeq Adapter, Index 1 (96% over 28bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT7830.6065911591081637TruSeq Adapter, Index 1 (96% over 26bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7820.6058164577555352No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAGGCAT5520.427635146650966No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC4530.3509397127407385No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC4370.33854449109868145No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC3570.2765683828883965Illumina PCR Primer Index 5 (95% over 24bp)
CTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC3520.2726948761252537No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3510.27192017477262515No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3470.2688213693621109No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3260.2525526409569111No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA2340.18128011651508344RNA PCR Primer, Index 1 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGAACTCGTATGCCGT2120.16423668675725508No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2090.1619125826993694No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATATCGTATGCCGT2070.1603631799941123No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG2030.15726437458359804RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG1980.1533908678204552TruSeq Adapter, Index 3 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT1970.15261616646782664No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGACCTCGTATGCCGT1920.14874265970468384No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1820.14099564617839824No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1810.14022094482576966Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1810.14022094482576966Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGAGCTCGTATGCCGT1590.1231775150679413No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG1560.12085341101005563RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG1510.1169799042469128RNA PCR Primer, Index 33 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1490.1154305015416557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1470.11388109883639858No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.11233169613114144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1430.11078229342588433No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT1390.10768348801537009No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG1370.10613408531011295RNA PCR Primer, Index 17 (96% over 28bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG1320.10226057854697014RNA PCR Primer, Index 26 (100% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)