FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001420893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224354
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT24581.0955900050812555No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT9140.407391889603038RNA PCR Primer, Index 46 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT6800.30309243427797145RNA PCR Primer, Index 46 (95% over 23bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG5850.2607486383126666RNA PCR Primer, Index 46 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4570.203695944801519No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4100.18274690890289455No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT3170.14129456127370138RNA PCR Primer, Index 46 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGT3070.13683731959314296No Hit
GTATAGACAAGCTTTGTAGTGAAGTATAGTAGCAATAATTTCTGTACCTG2780.12391131871952361No Hit
GTACATGAGAATCCTTTTTTGACAAGCCTTGGAAAGCTGACACTGTCTCT2610.11633400786257432No Hit
GTATTAACAAATGGCAATGAGTAGAAAAGTTAACATGAAGATTTTAGAAG2550.11365966285423927No Hit
TCTATGTACTGGGCAATTATATGCCAAACACTGCTGGGTGCCGGAGATAG2440.10875669700562504No Hit
CACTTAGACTATGCACTTTTTCTGAAATATTTTTGTAACACTTTTTTGTA2350.10474517949312248No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)