FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14593
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT295820.26999246213938No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT10196.982799972589598TruSeq Adapter, Index 11 (95% over 21bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG9696.640169944493936TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT7024.8105255944630985RNA PCR Primer, Index 11 (95% over 23bp)
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA3982.727335023641472RNA PCR Primer, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGT3442.3572945932981564No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT3262.2339477831837184TruSeq Adapter, Index 11 (96% over 26bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG1571.0758582882203798TruSeq Adapter, Index 11 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGT1330.9113958747344617No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC1230.8428698691153292No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTT960.6578496539436717No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC930.637291852257932No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT920.6304392516960187No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGT880.6030288494483657No Hit
TCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTCTGCTTG760.5207976427054067Illumina PCR Primer Index 9 (96% over 26bp)
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC700.4796820393339272RNA PCR Primer, Index 17 (95% over 24bp)
CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC600.41115603371479476No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGTCTT500.34263002809566234No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTCT490.33577742753374906Illumina PCR Primer Index 9 (95% over 21bp)
TCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAA460.31521962584800933TruSeq Adapter, Index 11 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.2809566230384431No Hit
CTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC400.2741040224765299No Hit
CTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTCTGCTTGA390.2672514219146166Illumina PCR Primer Index 9 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGT390.2672514219146166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.2535462207907901No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACTA360.24669362022887686No Hit
TCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGTCTTCTGCTTG360.24669362022887686Illumina Single End Adapter 1 (96% over 27bp)
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTT310.21243061741931063TruSeq Adapter, Index 11 (96% over 27bp)
CATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTCTGCT290.19872541629548413Illumina PCR Primer Index 9 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGTCTTCT280.1918728157335709Illumina Single End Adapter 1 (95% over 22bp)
TCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGTCTTCTGCTTG210.1439046118001782Illumina PCR Primer Index 9 (96% over 26bp)
GTACATGGGAGTGGGCTGCAACTCCTGTACTTGAAGCTGAGACCTCATAT210.1439046118001782No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGTCTT200.13705201123826494No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCGTACTA200.13705201123826494No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACT190.13019941067635168No Hit
GTTCTGTTCACTTGCAAACCATGGCTAAGGTCAAACTTGTAGAAAATAAA160.10964160899061194No Hit
CATATGACGTGGCCTTCGTGTTGTCAGAGAGTGTCTGGAAGCTGCTGTTG150.10278900842869869No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)