FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001420897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336327
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT35451.0540337231325465No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT11990.35649828886767937RNA PCR Primer, Index 17 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11590.34460510158268587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10050.298816330535461No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG8620.25629818599160936RNA PCR Primer, Index 17 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT8490.25243290012398645RNA PCR Primer, Index 17 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAACTCGTATGCCGT5440.16174734707591124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4620.13736631314167463No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT4040.1201211915784341RNA PCR Primer, Index 17 (96% over 26bp)
TGCATACACAGCTCAATGGGTCACCTGGAACAAACTTGCTTGACTATATT3620.10763334492919092No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)