FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001420901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806041
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT59340.7361908389275483No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31590.39191554772027726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26880.33348179559104313No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT20000.24812633600524042RNA PCR Primer, Index 27 (95% over 23bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG15040.1865910046759408RNA PCR Primer, Index 27 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT14810.18373755181188053RNA PCR Primer, Index 27 (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12510.15520302317127788No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)