FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30523
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT419313.73718179733316No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT14694.81276414507093RNA PCR Primer, Index 23 (95% over 23bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG13984.580152671755725RNA PCR Primer, Index 23 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT10563.4596861383219215RNA PCR Primer, Index 23 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGT7032.3031812076139304No Hit
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA5101.6708711463486552RNA PCR Primer, Index 23 (96% over 30bp)
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT4181.3694590964190938RNA PCR Primer, Index 23 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG2820.9238934573927857Illumina PCR Primer Index 1 (95% over 23bp)
GTATATGGGGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA2810.9206172394587687No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGT2520.8256069193722767No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTT2180.7142155096156997No Hit
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC1630.5340235232447662No Hit
TCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCTGCTTG1520.49798512597057953Illumina Single End Adapter 1 (96% over 25bp)
GAGTATATGGGGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCC1420.46522294663040986No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCT1390.45539429282835897No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGACCTCGTATGCCGT1370.448841856960325No Hit
ACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGC1350.442289421092291Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.4193558955541723No Hit
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT1260.41280345968613835RNA PCR Primer, Index 23 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.3767650624119516No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA1060.3472791010057989No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC950.3112407037316122No Hit
CTTTACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC930.3046882678635783RNA PCR Primer, Index 23 (95% over 21bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAGAGAG930.3046882678635783No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT910.29813583199554433No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTA900.2948596140615274No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTT790.2588212167873407No Hit
GTATCAACGCAGAGTATATGGGGGCGACAAACCTACCGAGCCTGGTGATA730.23916390918323885No Hit
CTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCTGCTTGA710.23261147331520493Illumina Single End Adapter 1 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATATCGTATGCCGT710.23261147331520493No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT700.22933525538118793No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG670.219506601579137No Hit
GGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAG650.2129541657111031No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTT640.20967794777708615No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT640.20967794777708615No Hit
CTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACT610.19984929397503523No Hit
TCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTTCTGCTTG610.19984929397503523Illumina Single End Adapter 1 (100% over 25bp)
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC580.1900206401729843No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC560.18346820430495037No Hit
ATATGGGGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGA560.18346820430495037No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA540.17691576843691642No Hit
TCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGAA540.17691576843691642Illumina PCR Primer Index 1 (96% over 27bp)
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC530.17363955050289945No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG530.17363955050289945No Hit
CTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC530.17363955050289945No Hit
GGTATCAACGCAGAGTATATGGGGGCGACAAACCTACCGAGCCTGGTGAT530.17363955050289945No Hit
GTACATGGGCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA530.17363955050289945No Hit
TGCCTGTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTG500.16381089670084853No Hit
GTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTGTAGAT490.16053467876683156No Hit
AACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC490.16053467876683156No Hit
ATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTT490.16053467876683156Illumina PCR Primer Index 1 (96% over 26bp)
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC490.16053467876683156No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC480.1572584608328146No Hit
GAGCATGCCTGTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTT470.15398224289879764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.15398224289879764No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCTGCT470.15398224289879764Illumina Single End Adapter 1 (95% over 23bp)
GCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTT440.14415358909674672No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTTCT440.14415358909674672No Hit
GGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCT440.14415358909674672No Hit
TATCAACGCAGAGTATATGGGGGCGACAAACCTACCGAGCCTGGTGATAG430.14087737116272975No Hit
ACGCAGAGTATATGGGGGCGACAAACCTACCGAGCCTGGTGATAGCTGGT420.13760115322871277No Hit
TTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTA420.13760115322871277No Hit
TCTCTCTACTGATCAACAGACTACATTTTCACAACTCTTCTTTCCTTTAC410.1343249352946958No Hit
CCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTT410.1343249352946958No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCAGAGAG410.1343249352946958No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA400.13104871736067883No Hit
GGCTTATGCAGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC400.13104871736067883No Hit
CTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTTCTGCTTGA400.13104871736067883Illumina Single End Adapter 1 (100% over 25bp)
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA400.13104871736067883No Hit
GTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAA390.12777249942666186No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT380.12449628149264488No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG380.12449628149264488No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT380.12449628149264488No Hit
TTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGC370.12122006355862792No Hit
TCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGG370.12122006355862792No Hit
GTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGG370.12122006355862792No Hit
GTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGAT350.11466762769059397No Hit
GTATCAACGCAGAGTACATGGGAATATACAAGTTTTCTTTAAAGCCCTCT350.11466762769059397No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCAGAGGAG350.11466762769059397No Hit
GGTTAGAAAGGTTTATTTTGTGCTTAGGATATTTTTCTAAGGCATCACAA340.111391409756577No Hit
CACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCTG340.111391409756577RNA PCR Primer, Index 23 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG340.111391409756577No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG340.111391409756577No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAGAGA330.10811519182256005No Hit
TCTCCGAGCCCACGAGACCAGAGAGGACCTCGTATGCCGTCTTCTGCTTG330.10811519182256005Illumina Single End Adapter 1 (96% over 25bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGACCTCGTATGCCGTCTTCT330.10811519182256005No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA330.10811519182256005No Hit
GTACATGGGAATATACAAGTTTTCTTTAAAGCCCTCTCCTTTAGAATTTA320.10483897388854307No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGACCTCGTATGCCGTCTT310.10156275595452609No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA310.10156275595452609No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)